Title |
Isolation and functional characterization of a τ-cadinol synthase, a new sesquiterpene synthase from Lavandula angustifolia
|
---|---|
Published in |
Plant Molecular Biology, September 2013
|
DOI | 10.1007/s11103-013-0131-3 |
Pubmed ID | |
Authors |
Frédéric Jullien, Sandrine Moja, Aurélie Bony, Sylvain Legrand, Cécile Petit, Tarek Benabdelkader, Kévin Poirot, Sébastien Fiorucci, Yann Guitton, Florence Nicolè, Sylvie Baudino, Jean-Louis Magnard |
Abstract |
In this paper we characterize three sTPSs: a germacrene D (LaGERDS), a (E)-β-caryophyllene (LaCARS) and a τ-cadinol synthase (LaCADS). τ-cadinol synthase is reported here for the first time and its activity was studied in several biological models including transiently or stably transformed tobacco species. Three dimensional structure models of LaCADS and Ocimum basilicum γ-cadinene synthase were built by homology modeling using the template structure of Gossypium arboreum δ-cadinene synthase. The depiction of their active site organization provides evidence of the global influence of the enzymes on the formation of τ-cadinol: instead of a unique amino-acid, the electrostatic properties and solvent accessibility of the whole active site in LaCADS may explain the stabilization of the cadinyl cation intermediate. Quantitative PCR performed from leaves and inflorescences showed two patterns of expression. LaGERDS and LaCARS were mainly expressed during early stages of flower development and, at these stages, transcript levels paralleled the accumulation of the corresponding terpene products (germacrene D and (E)-β-caryophyllene). By contrast, the expression level of LaCADS was constant in leaves and flowers. Phylogenetic analysis provided informative results on potential duplication process leading to sTPS diversification in lavender. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | 2% |
Serbia | 1 | 2% |
Unknown | 57 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 15 | 25% |
Researcher | 10 | 17% |
Professor > Associate Professor | 5 | 8% |
Student > Doctoral Student | 4 | 7% |
Student > Master | 4 | 7% |
Other | 11 | 19% |
Unknown | 10 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 26 | 44% |
Biochemistry, Genetics and Molecular Biology | 14 | 24% |
Chemistry | 3 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Unspecified | 1 | 2% |
Other | 2 | 3% |
Unknown | 12 | 20% |