| Title |
High-efficiency system for construction and evaluation of customized TALENs for silkworm genome editing
|
|---|---|
| Published in |
Molecular Genetics and Genomics, September 2013
|
| DOI | 10.1007/s00438-013-0782-4 |
| Pubmed ID | |
| Authors |
Feng Wang, Sanyuan Ma, Hanfu Xu, Jianping Duan, Yuancheng Wang, Huan Ding, Yuanyuan Liu, Xiaogang Wang, Ping Zhao, Qingyou Xia |
| Abstract |
Transcription activator-like effector nuclease (TALEN) possesses the characteristics of ease design and precise DNA targeting. In the silkworm Bombyx mori, TALEN has been successfully used to knockout an endogenous Bombyx gene, and shown the huge potential in functional genes research and improvement of the economical characteristics of silkworm. Thus, there is an urgent need to develop an applicable system that permits the efficient construction of customized TALEN with high activity that could efficiently induce the hereditable mutagenesis in the silkworm. In this study, we constructed an efficient assembly and evaluation system of the customized TALEN especiallly for silkworm genome editing by combination of a modified Golden Gate ligation strategy, a luciferase (LUC) reporter system in insect cell culture for binding activity and a surveyor nuclease assay system in silkworm embryos for cleavage efficiency. We showed the reliability of this system by assembling a pair of TALENs targeting a silkworm genome locus and assaying their binding and cleavage activities. The assembly strategy was convenient and efficient which allows the rapid construction of customized TALEN in less than 1 week, and the evaluation system was reliable and necessary for screening of the customized TALEN pair with high binding and cleavage activities. The results showed this system is a reliable and efficient tool for the construction of customized TALEN with high activity for gene targeting of silkworm, and will contribute to the wide application of TALEN technology in the functional gene research of silkworm. |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 2 | 6% |
| Netherlands | 1 | 3% |
| Unknown | 28 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 23% |
| Student > Master | 4 | 13% |
| Student > Postgraduate | 3 | 10% |
| Professor > Associate Professor | 3 | 10% |
| Unspecified | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 10 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 42% |
| Biochemistry, Genetics and Molecular Biology | 5 | 16% |
| Immunology and Microbiology | 1 | 3% |
| Unknown | 12 | 39% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 September 2013.
All research outputs
#6,741,509
of 25,374,917 outputs
Outputs from Molecular Genetics and Genomics
#822
of 3,319 outputs
Outputs of similar age
#55,275
of 217,316 outputs
Outputs of similar age from Molecular Genetics and Genomics
#1
of 3 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 3,319 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done well, scoring higher than 75% of its peers.
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We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them