| Title |
High Expression of H3K27me3 in Human Hepatocellular Carcinomas Correlates Closely with Vascular Invasion and Predicts Worse Prognosis in Patients
|
|---|---|
| Published in |
Molecular Medicine, September 2010
|
| DOI | 10.2119/molmed.2010.00103 |
| Pubmed ID | |
| Authors |
Mu-Yan Cai, Jing-Hui Hou, Hui-Lan Rao, Rong-Zhen Luo, Mei Li, Xiao-Qing Pei, Marie C. Lin, Xin-Yuan Guan, Hsiang-Fu Kung, Yi-Xin Zeng, Dan Xie |
| Abstract |
It has been suggested that trimethylation of lysine 27 on histone H3 (H3K27me3) is a crucial epigenetic process in tumorigenesis. However, the expression dynamics of H3K27me3 and its clinicopathological/prognostic significance in hepatocellular carcinoma (HCC) are unclear. In this study, immunohistochemical analysis (IHC) was used to examine protein expression of H3K27me3 in HCC tissues from two independent cohorts and corresponding nontumorous hepatocellular tissues by tissue microarray. The optimal cutpoint of H3K27me3 expression was assessed by the X-tile program. Our results showed that the cutpoint for high expression of H3K27me3 in HCCs was determined when more than 70% of the tumor cells showed positive staining. High expression of H3K27me3 was observed in 134 of 212 (63.2%) and 76 of 126 (60.4%) of HCCs in the testing and validation cohorts, respectively. Correlation analysis demonstrated that high expression of H3K27me3 in HCCs was significantly correlated with large tumor size, multiplicity, poor differentiation, advanced clinical stage and vascular invasion (P < 0.05). In addition, high expression of H3K27me3 in HCC patients was associated closely with shortened survival time, independent of serum α-fetoprotein levels, tumor size and multiplicity, clinical stage, vascular invasion and relapse as evidenced by univariate and multivariate analysis in both cohorts (P < 0.05). In different subsets of HCC patients, H3K27me3 expression was also a prognostic indicator in patients with stage II tumors (P < 0.05). Thus, these findings provide evidence that a high expression of H3K27me3, as detected by IHC, correlates closely with vascular invasion of HCCs and is an independent molecular marker for poor prognosis in patients with HCC. |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 4% |
| Japan | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 43 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 21% |
| Student > Ph. D. Student | 9 | 19% |
| Student > Doctoral Student | 7 | 15% |
| Other | 2 | 4% |
| Student > Postgraduate | 2 | 4% |
| Other | 6 | 13% |
| Unknown | 11 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 15 | 32% |
| Agricultural and Biological Sciences | 12 | 26% |
| Biochemistry, Genetics and Molecular Biology | 10 | 21% |
| Engineering | 2 | 4% |
| Physics and Astronomy | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 7 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2016.
All research outputs
#7,523,962
of 22,961,203 outputs
Outputs from Molecular Medicine
#368
of 1,145 outputs
Outputs of similar age
#34,492
of 95,788 outputs
Outputs of similar age from Molecular Medicine
#4
of 6 outputs
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