| Title |
Increased sensitivity of NS1 ELISA by heat dissociation in acute dengue 4 cases
|
|---|---|
| Published in |
BMC Infectious Diseases, March 2017
|
| DOI | 10.1186/s12879-017-2306-z |
| Pubmed ID | |
| Authors |
Sibelle Nogueira Buonora, Flavia Barreto dos Santos, Regina Paiva Daumas, Sonia Regina Lambert Passos, Manoela Heringer da Silva, Monique Rocha de Lima, Rita Maria Ribeiro Nogueira |
| Abstract |
Dengue is an acute febrile illness considered the major arboviral disease in terms of morbidity, mortality, economic impact and dissemination worldwide. Brazil accounts for the highest notification rate, with circulation of all four dengue serotypes. The NS1 antigen is a dengue highly conserved specific soluble glycoprotein essential for viral replication and viability that can be detected 0 to 18 days from the onset of fever (peak first 3 days). It induces a strong humoral response and is known as a complement-fixing antigen. Lower NS1 test sensitivity occurs in secondary dengue infections probably due to immune complex formation impairing antigen detection by ELISA. We compared the sensitivity of NS1 ELISA in heat dissociated and non-dissociated samples from 156 RT-PCR confirmed acute dengue-4 cases from 362 prospectively enrolled patients. Secondary infections accounted for 83.3% of cases. NS1 ELISA was positive in 42.5% and indeterminate in 10.2% of dengue-4 cases. After heat dissociation, 7 negative and 16 indeterminate samples turned positive, increasing the overall test sensitivity to 57.7%. Although it is time consuming and requires the use of specific laboratory equipment, NS1 ELISA combined with heat dissociation could be a slightly better alternative for triage in suspected dengue cases. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 56 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 56 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 11 | 20% |
| Researcher | 9 | 16% |
| Student > Bachelor | 7 | 13% |
| Student > Postgraduate | 3 | 5% |
| Student > Ph. D. Student | 3 | 5% |
| Other | 7 | 13% |
| Unknown | 16 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 11 | 20% |
| Biochemistry, Genetics and Molecular Biology | 7 | 13% |
| Social Sciences | 5 | 9% |
| Agricultural and Biological Sciences | 4 | 7% |
| Nursing and Health Professions | 2 | 4% |
| Other | 9 | 16% |
| Unknown | 18 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 July 2017.
All research outputs
#17,884,576
of 22,961,203 outputs
Outputs from BMC Infectious Diseases
#5,149
of 7,707 outputs
Outputs of similar age
#221,433
of 308,255 outputs
Outputs of similar age from BMC Infectious Diseases
#116
of 168 outputs
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