| Title |
miR-34 is associated with poor prognosis of patients with gallbladder cancer through regulating telomere length in tumor stem cells
|
|---|---|
| Published in |
Tumor Biology, September 2013
|
| DOI | 10.1007/s13277-013-1207-z |
| Pubmed ID | |
| Authors |
Ke Jin, Yonghua Xiang, Jing Tang, Guangchun Wu, Junwei Li, Huaichun Xiao, Chunwang Li, Yuxiang Chen, Jingfeng Zhao |
| Abstract |
miR-34a has been identified as a tumor suppressor in several tumors, but its involvement in gallbladder cancer (GBC) has not been reported. In this study, the miR-34a level and telomere length were measured in 77 gallbladder adenocarcinomas and 36 peritumoral tissues by real-time PCR. Forced miR-34a expression was established by an adenovirus carrying a miR-34a expression cassette. The colony-forming ability of isolated CD44+CD133+ GBC tumor stem-like cells was measured by matrigel colony assay. The xenograft tumor models were established by inoculating nude mice with CD44+CD133+cells. Results showed that significantly lower miR-34a expression and longer telomere length were observed in gallbladder adenocarcinoma tissues, which correlated with poor prognosis of GBC patients. Forced overexpression of miR-34a inhibited the colony-forming ability of CD44+CD133+ GBC tumor stem-like cells in vitro and xenograft tumor growth in vivo. Injection of Ad-miR-34a downregulated PNUTS expression and reduced telomere length in xenograft GBC tumor cells. In conclusion, miR-34a is a tumor suppressor in gallbladder cancer. Both low miR-34a expression and long telomere length are markers for poor prognosis of patients with gallbladder adenocarcinoma. Our study also suggests that the miR-34a gene could be a target for targeting therapy of GBC. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 22% |
| Student > Bachelor | 7 | 19% |
| Student > Master | 5 | 14% |
| Student > Ph. D. Student | 3 | 8% |
| Student > Postgraduate | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 9 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 30% |
| Biochemistry, Genetics and Molecular Biology | 8 | 22% |
| Medicine and Dentistry | 6 | 16% |
| Sports and Recreations | 1 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 10 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 October 2013.
All research outputs
#20,205,224
of 22,725,280 outputs
Outputs from Tumor Biology
#1,834
of 2,622 outputs
Outputs of similar age
#179,250
of 204,835 outputs
Outputs of similar age from Tumor Biology
#40
of 49 outputs
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