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Real-Time PCR Identification of Six Malassezia Species

Overview of attention for article published in Current Microbiology, March 2017
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  • Good Attention Score compared to outputs of the same age (67th percentile)
  • Good Attention Score compared to outputs of the same age and source (76th percentile)

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3 X users
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1 patent

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36 Mendeley
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Title
Real-Time PCR Identification of Six Malassezia Species
Published in
Current Microbiology, March 2017
DOI 10.1007/s00284-017-1237-7
Pubmed ID
Authors

Amin Ilahi, Inès Hadrich, Sourour Neji, Houaida Trabelsi, Fattouma Makni, Ali Ayadi

Abstract

Lipophilic yeast Malassezia species is widely found on the skin surface of humans and other animals. This fungus can cause pityriasis versicolor, Malassezia folliculitis, and seborrheic dermatitis. Still now, there is a problem with species identification of Malassezia with conventional methods. We developed a real-time polymerase chain reaction (PCR) assay with multiple hybridization probes for detecting M. globosa, M. furfur, M. restricta, M. sympodialis, M. slooffiae, and M. pachydermatis. The amplification curves and specific melting peaks of the probes hybridized with real-time PCR product were used for species identifications. The assay was further evaluated on 120 samples which were performed by swabbing from 60 domestic animals (23 goats, 10 dogs, 15 cows, 3 cats, 8 rabbits, and 1 donkey) and in 70 human samples (28 patients with pityriasis versicolor, 17 breeders, and 25 control group). Fifteen M. pachydermatis were identified from animals. From human, 61 isolates were identified as M. globosa (28), M. furfur (15), M. restricta (6), M. sympodialis (8), M. slooffiae (2), and M. pachydermatis (2). Eight cases of co-detection from 6 patients and 2 breeders were revealed. Our findings show that the assay was highly effective in identifying Malassezia species. The application of multiplex real-time PCR provides a sensitive and rapid identification system for Malassezia species, which may be applied in further epidemiological surveys from clinical samples.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Singapore 1 3%
Unknown 35 97%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 22%
Student > Master 4 11%
Student > Ph. D. Student 2 6%
Lecturer 2 6%
Student > Bachelor 2 6%
Other 8 22%
Unknown 10 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 19%
Biochemistry, Genetics and Molecular Biology 6 17%
Immunology and Microbiology 6 17%
Medicine and Dentistry 2 6%
Nursing and Health Professions 1 3%
Other 3 8%
Unknown 11 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 June 2022.
All research outputs
#6,199,434
of 22,961,203 outputs
Outputs from Current Microbiology
#397
of 2,421 outputs
Outputs of similar age
#100,171
of 309,336 outputs
Outputs of similar age from Current Microbiology
#7
of 30 outputs
Altmetric has tracked 22,961,203 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 2,421 research outputs from this source. They receive a mean Attention Score of 3.2. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,336 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.