Title |
The Human Immunodeficiency Virus 1 ASP RNA promotes viral latency by recruiting the Polycomb Repressor Complex 2 and promoting nucleosome assembly
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Published in |
Virology, March 2017
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DOI | 10.1016/j.virol.2017.03.002 |
Pubmed ID | |
Authors |
Juan C. Zapata, Federica Campilongo, Robert A. Barclay, Catherine DeMarino, Maria D. Iglesias-Ussel, Fatah Kashanchi, Fabio Romerio |
Abstract |
Various epigenetic marks at the HIV-1 5'LTR suppress proviral expression and promote latency. Cellular antisense transcripts known as long noncoding RNAs (lncRNAs) recruit the polycomb repressor complex 2 (PRC2) to gene promoters, which catalyzes trimethylation of lysine 27 on histone H3 (H3K27me3), thus promoting nucleosome assembly and suppressing gene expression. We found that an HIV-1 antisense transcript expressed from the 3'LTR and encoding the antisense protein ASP promotes proviral latency. Expression of ASP RNA reduced HIV-1 replication in Jurkat cells. Moreover, ASP RNA expression promoted the establishment and maintenance of HIV-1 latency in Jurkat E4 cells. We show that this transcript interacts with and recruits PRC2 to the HIV-1 5'LTR, increasing accumulation of the suppressive epigenetic mark H3K27me3, while reducing RNA Polymerase II and thus proviral transcription. Altogether, our results suggest that the HIV-1 ASP transcript promotes epigenetic silencing of the HIV-1 5'LTR and proviral latency through the PRC2 pathway. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 61 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 14 | 23% |
Student > Master | 9 | 15% |
Student > Bachelor | 8 | 13% |
Researcher | 7 | 11% |
Student > Doctoral Student | 4 | 7% |
Other | 4 | 7% |
Unknown | 15 | 25% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 16 | 26% |
Agricultural and Biological Sciences | 16 | 26% |
Immunology and Microbiology | 6 | 10% |
Medicine and Dentistry | 4 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 2 | 3% |
Unknown | 16 | 26% |