Title |
MiR-9 enhances the sensitivity of A549 cells to cisplatin by inhibiting autophagy
|
---|---|
Published in |
Biotechnology Techniques, March 2017
|
DOI | 10.1007/s10529-017-2325-2 |
Pubmed ID | |
Authors |
Yan Zhang, Xia Meng, Cheng Li, Zhoulin Tan, Xinwei Guo, Zhiting Zhang, Tao Xi |
Abstract |
To demonstrate that miR-9 inhibits autophagy by down-regulating Beclin1 and thus enhances the sensitivity of A549 cells to cisplatin. MiR-9 inhibited Beclin1 expression by binding to its 3'UTR. The inhibition decreased the cisplatin-induced autophagy in A549 cells, evidenced by the decreased expression of LC3II and GFP-LC3 puncta and the increased expression of P62. Upregulation of miR-9 level enhanced the sensibility of A549 cells to cisplatin and increased the cisplatin-induced apoptosis. Overexpression of Beclin1 reversed above effects of miR-9 mimics, cisplatin-induced autophagy was increased and apoptosis was decreased. MiR-9 inhibits autophagy via targeting Beclin1 3'UTR and thus enhances cisplatin sensitivity in A549 cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 14 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 36% |
Student > Bachelor | 2 | 14% |
Student > Master | 2 | 14% |
Researcher | 1 | 7% |
Unknown | 4 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 43% |
Medicine and Dentistry | 3 | 21% |
Unknown | 5 | 36% |