| Title |
A High Resolution/Accurate Mass (HRAM) Data-Dependent MS3 Neutral Loss Screening, Classification, and Relative Quantitation Methodology for Carbonyl Compounds in Saliva
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, October 2016
|
| DOI | 10.1007/s13361-016-1521-y |
| Pubmed ID | |
| Authors |
Romel Dator, Andrea Carrà, Laura Maertens, Valeria Guidolin, Peter W. Villalta, Silvia Balbo |
| Abstract |
Reactive carbonyl compounds (RCCs) are ubiquitous in the environment and are generated endogenously as a result of various physiological and pathological processes. These compounds can react with biological molecules inducing deleterious processes believed to be at the basis of their toxic effects. Several of these compounds are implicated in neurotoxic processes, aging disorders, and cancer. Therefore, a method characterizing exposures to these chemicals will provide insights into how they may influence overall health and contribute to disease pathogenesis. Here, we have developed a high resolution accurate mass (HRAM) screening strategy allowing simultaneous identification and relative quantitation of DNPH-derivatized carbonyls in human biological fluids. The screening strategy involves the diagnostic neutral loss of hydroxyl radical triggering MS(3) fragmentation, which is only observed in positive ionization mode of DNPH-derivatized carbonyls. Unique fragmentation pathways were used to develop a classification scheme for characterizing known and unanticipated/unknown carbonyl compounds present in saliva. Furthermore, a relative quantitation strategy was implemented to assess variations in the levels of carbonyl compounds before and after exposure using deuterated d 3 -DNPH. This relative quantitation method was tested on human samples before and after exposure to specific amounts of alcohol. The nano-electrospray ionization (nano-ESI) in positive mode afforded excellent sensitivity with detection limits on-column in the high-attomole levels. To the best of our knowledge, this is the first report of a method using HRAM neutral loss screening of carbonyl compounds. In addition, the method allows simultaneous characterization and relative quantitation of DNPH-derivatized compounds using nano-ESI in positive mode. Graphical Abstract ᅟ. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 33% |
| United States | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 33% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
| Scientists | 1 | 33% |
Mendeley readers
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 22% |
| Other | 4 | 17% |
| Researcher | 2 | 9% |
| Student > Bachelor | 2 | 9% |
| Librarian | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 6 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Environmental Science | 3 | 13% |
| Biochemistry, Genetics and Molecular Biology | 3 | 13% |
| Chemistry | 3 | 13% |
| Computer Science | 2 | 9% |
| Engineering | 2 | 9% |
| Other | 3 | 13% |
| Unknown | 7 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2017.
All research outputs
#19,945,185
of 25,374,917 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#2,945
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Outputs of similar age
#234,269
of 321,047 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#32
of 57 outputs
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