| Title |
CRISPR-Cas9 and CRISPR-Cpf1 mediated targeting of a stomatal developmental gene EPFL9 in rice
|
|---|---|
| Published in |
Plant Cell Reports, March 2017
|
| DOI | 10.1007/s00299-017-2118-z |
| Pubmed ID | |
| Authors |
Xiaojia Yin, Akshaya K. Biswal, Jacqueline Dionora, Kristel M. Perdigon, Christian P. Balahadia, Shamik Mazumdar, Caspar Chater, Hsiang-Chun Lin, Robert A. Coe, Tobias Kretzschmar, Julie E. Gray, Paul W. Quick, Anindya Bandyopadhyay |
| Abstract |
CRISPR-Cas9/Cpf1 system with its unique gene targeting efficiency, could be an important tool for functional study of early developmental genes through the generation of successful knockout plants. The introduction and utilization of systems biology approaches have identified several genes that are involved in early development of a plant and with such knowledge a robust tool is required for the functional validation of putative candidate genes thus obtained. The development of the CRISPR-Cas9/Cpf1 genome editing system has provided a convenient tool for creating loss of function mutants for genes of interest. The present study utilized CRISPR/Cas9 and CRISPR-Cpf1 technology to knock out an early developmental gene EPFL9 (Epidermal Patterning Factor like-9, a positive regulator of stomatal development in Arabidopsis) orthologue in rice. Germ-line mutants that were generated showed edits that were carried forward into the T2 generation when Cas9-free homozygous mutants were obtained. The homozygous mutant plants showed more than an eightfold reduction in stomatal density on the abaxial leaf surface of the edited rice plants. Potential off-target analysis showed no significant off-target effects. This study also utilized the CRISPR-LbCpf1 (Lachnospiracae bacterium Cpf1) to target the same OsEPFL9 gene to test the activity of this class-2 CRISPR system in rice and found that Cpf1 is also capable of genome editing and edits get transmitted through generations with similar phenotypic changes seen with CRISPR-Cas9. This study demonstrates the application of CRISPR-Cas9/Cpf1 to precisely target genomic locations and develop transgene-free homozygous heritable gene edits and confirms that the loss of function analysis of the candidate genes emerging from different systems biology based approaches, could be performed, and therefore, this system adds value in the validation of gene function studies. |
X Demographics
The data shown below were collected from the profiles of 13 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 15% |
| Mexico | 1 | 8% |
| Canada | 1 | 8% |
| India | 1 | 8% |
| Germany | 1 | 8% |
| Spain | 1 | 8% |
| Unknown | 6 | 46% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 7 | 54% |
| Scientists | 6 | 46% |
Mendeley readers
The data shown below were compiled from readership statistics for 237 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| United States | 1 | <1% |
| Sri Lanka | 1 | <1% |
| Argentina | 1 | <1% |
| Unknown | 233 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 51 | 22% |
| Researcher | 42 | 18% |
| Student > Master | 28 | 12% |
| Student > Bachelor | 22 | 9% |
| Student > Postgraduate | 12 | 5% |
| Other | 32 | 14% |
| Unknown | 50 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 110 | 46% |
| Biochemistry, Genetics and Molecular Biology | 55 | 23% |
| Computer Science | 4 | 2% |
| Engineering | 4 | 2% |
| Medicine and Dentistry | 3 | 1% |
| Other | 13 | 5% |
| Unknown | 48 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2023.
All research outputs
#3,330,337
of 24,954,788 outputs
Outputs from Plant Cell Reports
#134
of 2,325 outputs
Outputs of similar age
#58,264
of 314,456 outputs
Outputs of similar age from Plant Cell Reports
#2
of 46 outputs
Altmetric has tracked 24,954,788 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,325 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done particularly well, scoring higher than 94% of its peers.
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We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.