| Title |
Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics
|
|---|---|
| Published in |
Biomedical Microdevices, March 2017
|
| DOI | 10.1007/s10544-017-0159-2 |
| Pubmed ID | |
| Authors |
G. Papadakis, J. M. Friedt, M. Eck, D. Rabus, G. Jobst, E. Gizeli |
| Abstract |
The development of integrated platforms incorporating an acoustic device as the detection element requires addressing simultaneously several challenges of technological and scientific nature. The present work was focused on the design of a microfluidic module, which, combined with a dual or array type Love wave acoustic chip could be applied to biomedical applications and molecular diagnostics. Based on a systematic study we optimized the mechanics of the flow cell attachment and the sealing material so that fluidic interfacing/encapsulation would impose minimal losses to the acoustic wave. We have also investigated combinations of operating frequencies with waveguide materials and thicknesses for maximum sensitivity during the detection of protein and DNA biomarkers. Within our investigations neutravidin was used as a model protein biomarker and unpurified PCR amplified Salmonella DNA as the model genetic target. Our results clearly indicate the need for experimental verification of the optimum engineering and analytical parameters, in order to develop commercially viable systems for integrated analysis. The good reproducibility of the signal together with the ability of the array biochip to detect multiple samples hold promise for the future use of the integrated system in a Lab-on-a-Chip platform for application to molecular diagnostics. |
Mendeley readers
The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 29 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 24% |
| Student > Ph. D. Student | 6 | 21% |
| Professor > Associate Professor | 3 | 10% |
| Student > Master | 3 | 10% |
| Student > Bachelor | 2 | 7% |
| Other | 5 | 17% |
| Unknown | 3 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 8 | 28% |
| Biochemistry, Genetics and Molecular Biology | 4 | 14% |
| Agricultural and Biological Sciences | 4 | 14% |
| Business, Management and Accounting | 1 | 3% |
| Unspecified | 1 | 3% |
| Other | 5 | 17% |
| Unknown | 6 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2018.
All research outputs
#7,688,781
of 23,393,513 outputs
Outputs from Biomedical Microdevices
#242
of 770 outputs
Outputs of similar age
#121,525
of 309,756 outputs
Outputs of similar age from Biomedical Microdevices
#5
of 11 outputs
Altmetric has tracked 23,393,513 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 770 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 32nd percentile – i.e., 32% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,756 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 11 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 63% of its contemporaries.