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Probiotics enhance pancreatic glutathione biosynthesis and reduce oxidative stress in experimental acute pancreatitis

Overview of attention for article published in American Journal of Physiology: Gastrointestinal & Liver Physiology, October 2008
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Title
Probiotics enhance pancreatic glutathione biosynthesis and reduce oxidative stress in experimental acute pancreatitis
Published in
American Journal of Physiology: Gastrointestinal & Liver Physiology, October 2008
DOI 10.1152/ajpgi.00603.2007
Pubmed ID
Authors

Femke Lutgendorff, Lena M. Trulsson, L. Paul van Minnen, Ger T. Rijkers, Harro M. Timmerman, Lennart E. Franzén, Hein G. Gooszen, Louis M. A. Akkermans, Johan D. Söderholm, Per A. Sandström

Abstract

Factors determining severity of acute pancreatitis (AP) are poorly understood. Oxidative stress causes acinar cell injury and contributes to the severity, whereas prophylactic probiotics ameliorate experimental pancreatitis. Our objective was to study how probiotics affect oxidative stress, inflammation, and acinar cell injury during the early phase of AP. Fifty-three male Sprague-Dawley rats were randomly allocated into groups: 1) control, 2) sham procedure, 3) AP with no treatment, 4) AP with probiotics, and 5) AP with placebo. AP was induced under general anesthesia by intraductal glycodeoxycholate infusion (15 mM) and intravenous cerulein (5 microg.kg(-1).h(-1), for 6 h). Daily probiotics or placebo were administered intragastrically, starting 5 days prior to AP. After cerulein infusion, pancreas samples were collected for analysis including lipid peroxidation, glutathione, glutamate-cysteine-ligase activity, histological grading of pancreatic injury, and NF-kappaB activation. The severity of pancreatic injury correlated to oxidative damage (r = 0.9) and was ameliorated by probiotics (1.5 vs. placebo 5.5; P = 0.014). AP-induced NF-kappaB activation was reduced by probiotics (0.20 vs. placebo 0.53 OD(450nm)/mg nuclear protein; P < 0.001). Probiotics attenuated AP-induced lipid peroxidation (0.25 vs. placebo 0.51 pmol malondialdehyde/mg protein; P < 0.001). Not only was AP-induced glutathione depletion prevented (8.81 vs. placebo 4.1 micromol/mg protein, P < 0.001), probiotic pretreatment even increased glutathione compared with sham rats (8.81 vs. sham 6.18 miccromol/mg protein, P < 0.001). Biosynthesis of glutathione (glutamate-cysteine-ligase activity) was enhanced in probiotic-pretreated animals. Probiotics enhanced the biosynthesis of glutathione, which may have reduced activation of inflammation and acinar cell injury and ameliorated experimental AP, via a reduction in oxidative stress.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Turkey 1 1%
Netherlands 1 1%
Unknown 79 98%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 13 16%
Researcher 12 15%
Student > Ph. D. Student 9 11%
Student > Master 8 10%
Student > Doctoral Student 6 7%
Other 16 20%
Unknown 17 21%
Readers by discipline Count As %
Medicine and Dentistry 17 21%
Agricultural and Biological Sciences 17 21%
Biochemistry, Genetics and Molecular Biology 9 11%
Immunology and Microbiology 6 7%
Nursing and Health Professions 4 5%
Other 10 12%
Unknown 18 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 January 2017.
All research outputs
#20,655,488
of 25,373,627 outputs
Outputs from American Journal of Physiology: Gastrointestinal & Liver Physiology
#1,922
of 2,218 outputs
Outputs of similar age
#93,680
of 101,411 outputs
Outputs of similar age from American Journal of Physiology: Gastrointestinal & Liver Physiology
#16
of 17 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,218 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one is in the 7th percentile – i.e., 7% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 101,411 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 3rd percentile – i.e., 3% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 17 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.