| Chapter title |
Reconstitution of a Patterned Neural Tube from Single Mouse Embryonic Stem Cells
|
|---|---|
| Chapter number | 4 |
| Book title |
Organ Regeneration
|
| Published in |
Methods in molecular biology, March 2017
|
| DOI | 10.1007/978-1-4939-6949-4_4 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6947-0, 978-1-4939-6949-4
|
| Authors |
Ishihara, Keisuke, Ranga, Adrian, Lutolf, Matthias P., Tanaka, Elly M., Meinhardt, Andrea, Keisuke Ishihara, Adrian Ranga, Matthias P. Lutolf, Elly M. Tanaka, Andrea Meinhardt |
| Editors |
Takashi Tsuji |
| Abstract |
The recapitulation of tissue development and patterning in three-dimensional (3D) culture is an important dimension of stem cell research. Here, we describe a 3D culture protocol in which single mouse ES cells embedded in Matrigel under neural induction conditions clonally form a lumen containing, oval-shaped epithelial structure within 3 days. By Day 7 an apicobasally polarized neuroepithelium with uniformly dorsal cell identity forms. Treatment with retinoic acid at Day 2 results in posteriorization and self-organization of dorsal-ventral neural tube patterning. Neural tube organoid growth is also supported by pure laminin gels as well as poly(ethylene glycol) (PEG)-based artificial extracellular matrix hydrogels, which can be fine-tuned for key microenvironment characteristics. The rapid generation of a simple, patterned tissue in well-defined culture conditions makes the neural tube organoid a tractable model for studying neural stem cell self-organization. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 52 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 17% |
| Researcher | 8 | 15% |
| Student > Bachelor | 7 | 13% |
| Student > Postgraduate | 4 | 8% |
| Student > Master | 4 | 8% |
| Other | 5 | 10% |
| Unknown | 15 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 25% |
| Biochemistry, Genetics and Molecular Biology | 10 | 19% |
| Medicine and Dentistry | 4 | 8% |
| Engineering | 3 | 6% |
| Neuroscience | 3 | 6% |
| Other | 3 | 6% |
| Unknown | 16 | 31% |