Title |
Identification of genome-wide single nucleotide polymorphisms in allopolyploid crop Brassica napus
|
---|---|
Published in |
BMC Genomics, October 2013
|
DOI | 10.1186/1471-2164-14-717 |
Pubmed ID | |
Authors |
Shunmou Huang, Linbin Deng, Mei Guan, Jiana Li, Kun Lu, Hanzhong Wang, Donghui Fu, Annaliese S Mason, Shengyi Liu, Wei Hua |
Abstract |
Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation. Identification of large numbers of SNPs is helpful for genetic diversity analysis, map-based cloning, genome-wide association analyses and marker-assisted breeding. Recently, identifying genome-wide SNPs in allopolyploid Brassica napus (rapeseed, canola) by resequencing many accessions has become feasible, due to the availability of reference genomes of Brassica rapa (2n = AA) and Brassica oleracea (2n = CC), which are the progenitor species of B. napus (2n = AACC). Although many SNPs in B. napus have been released, the objective in the present study was to produce a larger, more informative set of SNPs for large-scale and efficient genotypic screening. Hence, short-read genome sequencing was conducted on ten elite B. napus accessions for SNP discovery. A subset of these SNPs was randomly selected for sequence validation and for genotyping efficiency testing using the Illumina GoldenGate assay. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 79 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 20 | 25% |
Researcher | 19 | 24% |
Student > Master | 8 | 10% |
Student > Bachelor | 5 | 6% |
Professor > Associate Professor | 5 | 6% |
Other | 8 | 10% |
Unknown | 14 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 49 | 62% |
Biochemistry, Genetics and Molecular Biology | 9 | 11% |
Computer Science | 2 | 3% |
Unspecified | 1 | 1% |
Engineering | 1 | 1% |
Other | 0 | 0% |
Unknown | 17 | 22% |