| Title |
Hydroxylated Fluorescent Dyes for Live‐Cell Labeling: Synthesis, Spectra and Super‐Resolution STED
|
|---|---|
| Published in |
Chemistry - A European Journal, May 2017
|
| DOI | 10.1002/chem.201701216 |
| Pubmed ID | |
| Authors | |
| Abstract |
Hydroxylated rhodamines, carbopyronines, silico- and germanorhodamines with absorption maxima in the range of 530-640 nm were prepared and applied in specific labeling of living cells. The direct and high-yielding entry to germa- and silaxanthones tolerates the presence of protected heteroatoms and may be considered for the syntheses of various sila- and germafluoresceins, as well as -rhodols. Application in stimulated emission depletion (STED) fluorescence microscopy revealed a resolution of 50-75 nm in one- and two-color imaging of vimentin-HaloTag fused protein and native tubulin. The established structure-property relationships allow prediction of the spectral properties and the positions of spirolactone/zwitterion equilibria for the new analogs of rhodamines, carbo-, silico- and germanorhodamines using simple additive schemes. |
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X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 3 | 60% |
| Unknown | 2 | 40% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Scientists | 2 | 40% |
Mendeley demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 96 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 23% |
| Researcher | 16 | 17% |
| Student > Master | 12 | 13% |
| Student > Bachelor | 7 | 7% |
| Professor | 5 | 5% |
| Other | 16 | 17% |
| Unknown | 18 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 34 | 35% |
| Biochemistry, Genetics and Molecular Biology | 11 | 11% |
| Physics and Astronomy | 9 | 9% |
| Agricultural and Biological Sciences | 5 | 5% |
| Neuroscience | 2 | 2% |
| Other | 11 | 11% |
| Unknown | 24 | 25% |