| Title |
Electrical Stimulation Promotes Maturation of Cardiomyocytes Derived from Human Embryonic Stem Cells
|
|---|---|
| Published in |
Journal of Cardiovascular Translational Research, October 2013
|
| DOI | 10.1007/s12265-013-9510-z |
| Pubmed ID | |
| Authors |
Yau-Chi Chan, Sherwin Ting, Yee-Ki Lee, Kwong-Man Ng, Jiao Zhang, Zi Chen, Chung-Wah Siu, Steve K. W. Oh, Hung-Fat Tse |
| Abstract |
While human embryonic stem cells (hESCs) can differentiate into functional cardiomyocytes, their immature phenotypes limit their therapeutic application for myocardial regeneration. We sought to determine whether electrical stimulation could enhance the differentiation and maturation of hESC-derived cardiomyocytes. Cardiac differentiation was induced in a HES3 hESC line via embryoid bodies formation treated with a p38 MAP kinase inhibitor. Detailed molecular and functional analysis were performed in those hESC-derived cardiomyocytes cultured for 4 days in the absence or presence of electrical field stimulation (6.6 V/cm, 1 Hz, and 2 ms pulses) using an eight-channel C-Pace stimulator (Ion-Optics Co., MA). Upon electrical stimulation, quantitative polymerase chain reaction demonstrated significant upregulation of cardiac-specific gene expression including HCN1, MLC2V, SCN5A, SERCA, Kv4.3, and GATA4; immunostaining and flow cytometry analysis revealed cellular elongation and an increased proportion of troponin-T positive cells (6.3 ± 1.2% vs. 15.8 ± 2.1%; n = 3, P < 0.01). Electrophysiological studies showed an increase in the proportion of ventricular-like hESC-derived cardiomyocytes (48 vs. 29%, P < 0.05) with lengthening of their action potential duration at 90% repolarization (387.7 ± 35.35; n = 11 vs. 291.8 ± 20.82; n = 10, P < 0.05) and 50% repolarization (313.9 ± 27.94; n = 11 vs. 234.0 ± 16.10; n = 10, P < 0.05) after electrical stimulation. Nonetheless, the membrane diastolic potentials and action potential upstrokes of different hESC-derived cardiomyocyte phenotypes, and the overall beating rate remained unchanged (all P > 0.05). Fluorescence confocal imaging revealed that electrical stimulation significantly increased both spontaneous and caffeine-induced calcium flux in the hESC-derived cardiomyocytes (approximately 1.6-fold for both cases; P < 0.01). In conclusion, electrical field stimulation increased the expression of cardiac-specific genes and the yield of differentiation, promoted ventricular-like phenotypes, and improved the calcium handling of hESC-derived cardiomyocytes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 258 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | <1% |
| France | 1 | <1% |
| India | 1 | <1% |
| United Kingdom | 1 | <1% |
| United States | 1 | <1% |
| Unknown | 253 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 63 | 24% |
| Student > Master | 30 | 12% |
| Student > Bachelor | 28 | 11% |
| Researcher | 26 | 10% |
| Student > Doctoral Student | 16 | 6% |
| Other | 36 | 14% |
| Unknown | 59 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 44 | 17% |
| Agricultural and Biological Sciences | 39 | 15% |
| Engineering | 39 | 15% |
| Medicine and Dentistry | 28 | 11% |
| Materials Science | 8 | 3% |
| Other | 34 | 13% |
| Unknown | 66 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 October 2013.
All research outputs
#18,351,676
of 22,727,570 outputs
Outputs from Journal of Cardiovascular Translational Research
#433
of 575 outputs
Outputs of similar age
#154,372
of 207,109 outputs
Outputs of similar age from Journal of Cardiovascular Translational Research
#4
of 6 outputs
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