| Title |
NMR resonance assignments of an engineered neomycin-sensing riboswitch RNA bound to ribostamycin and tobramycin
|
|---|---|
| Published in |
Biomolecular NMR Assignments, March 2010
|
| DOI | 10.1007/s12104-010-9223-z |
| Pubmed ID | |
| Authors |
Sina R. Schmidtke, Elke Duchardt-Ferner, Julia E. Weigand, Beatrix Suess, Jens Wöhnert |
| Abstract |
The neomycin-sensing riboswitch is an engineered riboswitch developed to regulate gene expression in vivo in the lower eukaryote Saccharomyces cerevisiae upon binding to neomycin B. With a size of only 27nt it is the smallest functional riboswitch element identified so far. It binds not only neomycin B but also related aminoglycosides of the 2'-deoxystreptamine class with high affinity. The regulatory activity, however, strongly depends on the identity of the aminoglycoside. As a prerequisite for the structure determination of riboswitch-ligand complexes we report here the (1)H, (15)N, (13)C and partial (31)P chemical shift assignments for the minimal functional 27nt neomycin sensing riboswitch RNA in complex with the 4,5-linked neomycin analog ribostamycin and the 4,6-linked aminoglycoside tobramycin. |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 27% |
| Student > Ph. D. Student | 7 | 27% |
| Professor > Associate Professor | 3 | 12% |
| Student > Bachelor | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 1 | 4% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 42% |
| Chemistry | 8 | 31% |
| Biochemistry, Genetics and Molecular Biology | 2 | 8% |
| Environmental Science | 1 | 4% |
| Unknown | 4 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 October 2013.
All research outputs
#15,283,138
of 22,727,570 outputs
Outputs from Biomolecular NMR Assignments
#94
of 276 outputs
Outputs of similar age
#76,965
of 94,400 outputs
Outputs of similar age from Biomolecular NMR Assignments
#1
of 1 outputs
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So far Altmetric has tracked 276 research outputs from this source. They receive a mean Attention Score of 1.7. This one has gotten more attention than average, scoring higher than 56% of its peers.
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