| Title |
Chrysoeriol potently inhibits the induction of nitric oxide synthase by blocking AP-1 activation
|
|---|---|
| Published in |
Journal of Biomedical Science, October 2005
|
| DOI | 10.1007/s11373-005-9028-8 |
| Pubmed ID | |
| Authors |
Doo-Youn Choi, Jeong Yong Lee, Mi-Ran Kim, Eun-Rhan Woo, Yoon Gyoon Kim, Keon Wook Kang |
| Abstract |
Chrysoeriol is a flavonoid with antioxidant and anti-inflammatory activities. Despite the large number of studies performed on its biological activities, no clear picture of its mode of action has emerged. In the present study, we isolated chrysoeriol from the leaves of Digitalis purpurea (foxglove), and studied its effect on the induction of the inducible nitric oxide synthase (iNOS) gene, and the mechanism of this induction in Raw264.7 macrophages. Chrysoeriol pretreatment potently inhibited the release of NO in the cells treated with lipopolysaccharide (LPS), and Western blot and RT-PCR analyses revealed that chrysoeriol inhibited the LPS-induced inductions of iNOS gene. Moreover, it is known that the activations of nuclear factor-kappaB (NF-kappaB) and activator protein 1 (AP-1) are crucial steps in the transcriptional activation of the iNOS gene. Here, we found that chrysoeriol selectively suppressed AP-1 activation, and that activation of AP-1 is likely to be essential for iNOS induction in LPS-treated macrophages. This presumed inhibitory effect on AP-1 activation by chrysoeriol may be associated with its potent NO blocking and anti-inflammatory effects. |
Mendeley readers
The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| China | 1 | 5% |
| Australia | 1 | 5% |
| Unknown | 18 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 30% |
| Student > Ph. D. Student | 5 | 25% |
| Professor > Associate Professor | 2 | 10% |
| Student > Master | 2 | 10% |
| Student > Bachelor | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 2 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 20% |
| Biochemistry, Genetics and Molecular Biology | 4 | 20% |
| Medicine and Dentistry | 4 | 20% |
| Agricultural and Biological Sciences | 2 | 10% |
| Chemistry | 2 | 10% |
| Other | 1 | 5% |
| Unknown | 3 | 15% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 October 2013.
All research outputs
#5,847,332
of 22,727,570 outputs
Outputs from Journal of Biomedical Science
#237
of 981 outputs
Outputs of similar age
#17,320
of 58,511 outputs
Outputs of similar age from Journal of Biomedical Science
#2
of 13 outputs
Altmetric has tracked 22,727,570 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 981 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.3. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 58,511 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 13 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.