| Title |
Mitochondrial Ca2+ uniporter (MCU)-dependent and MCU-independent Ca2+ channels coexist in the inner mitochondrial membrane
|
|---|---|
| Published in |
Pflügers Archiv - European Journal of Physiology, October 2013
|
| DOI | 10.1007/s00424-013-1383-0 |
| Pubmed ID | |
| Authors |
Alexander I. Bondarenko, Claire Jean-Quartier, Warisara Parichatikanond, Muhammad Rizwan Alam, Markus Waldeck-Weiermair, Roland Malli, Wolfgang F. Graier |
| Abstract |
A protein referred to as CCDC109A and then renamed to mitochondrial calcium uniporter (MCU) has recently been shown to accomplish mitochondrial Ca(2+) uptake in different cell types. In this study, we investigated whole-mitoplast inward cation currents and single Ca(2+) channel activities in mitoplasts prepared from stable MCU knockdown HeLa cells using the patch-clamp technique. In whole-mitoplast configuration, diminution of MCU considerably reduced inward Ca(2+) and Na(+) currents. This was accompanied by a decrease in occurrence of single channel activity of the intermediate conductance mitochondrial Ca(2+) current (i-MCC). However, ablation of MCU yielded a compensatory 2.3-fold elevation in the occurrence of the extra large conductance mitochondrial Ca(2+) current (xl-MCC), while the occurrence of bursting currents (b-MCC) remained unaltered. These data reveal i-MCC as MCU-dependent current while xl-MCC and b-MCC seem to be rather MCU-independent, thus, pointing to the engagement of at least two molecularly distinct mitochondrial Ca(2+) channels. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 2% |
| Unknown | 48 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 31% |
| Student > Ph. D. Student | 8 | 16% |
| Student > Bachelor | 5 | 10% |
| Professor > Associate Professor | 4 | 8% |
| Student > Postgraduate | 3 | 6% |
| Other | 8 | 16% |
| Unknown | 6 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 17 | 35% |
| Agricultural and Biological Sciences | 11 | 22% |
| Medicine and Dentistry | 4 | 8% |
| Neuroscience | 4 | 8% |
| Chemistry | 3 | 6% |
| Other | 3 | 6% |
| Unknown | 7 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 January 2014.
All research outputs
#3,227,829
of 23,815,455 outputs
Outputs from Pflügers Archiv - European Journal of Physiology
#115
of 1,973 outputs
Outputs of similar age
#30,259
of 214,458 outputs
Outputs of similar age from Pflügers Archiv - European Journal of Physiology
#4
of 24 outputs
Altmetric has tracked 23,815,455 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,973 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done particularly well, scoring higher than 94% of its peers.
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We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.