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Fluorescence Spectroscopy and Microscopy

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Cover of 'Fluorescence Spectroscopy and Microscopy'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra
  3. Altmetric Badge
    Chapter 2 Steady-State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions
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    Chapter 3 Quantitative Fluorescence Spectral Analysis of Protein Denaturation
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    Chapter 4 High-Pressure Fluorescence Applications
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    Chapter 5 Frequency Domain Fluorometry: Theory and Application
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    Chapter 6 Polar Plot Representation of Time-Resolved Fluorescence
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    Chapter 7 Ensemble and Single-Molecule Detected Time-Resolved FRET Methods in Studies of Protein Conformations and Dynamics
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    Chapter 8 MD + QM Correlations with Tryptophan Fluorescence Spectral Shifts and Lifetimes
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    Chapter 9 Analysis of Time-Dependent Red Shifts in Fluorescence Emission from Tryptophan Residues in Proteins
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    Chapter 10 Global Analysis of Time-Resolved Fluorescence Data
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    Chapter 11 Nanometrology.
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    Chapter 12 Upconversion Spectrophotofluorometry
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    Chapter 13 Subpicosecond Kerr-Gate Spectrofluorometry
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    Chapter 14 Photoinduced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay
  16. Altmetric Badge
    Chapter 15 Biosynthetic Incorporation of Tryptophan Analogs in Proteins
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    Chapter 16 Optimization of Fluorescent Proteins
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    Chapter 17 Monitoring Membrane Properties and Apoptosis Using Membrane Probes of the 3-Hydroxyflavone Family
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    Chapter 18 Rectangle FRAP for Measuring Diffusion with a Laser Scanning Microscope
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    Chapter 19 A Quantitative Protocol for Intensity-Based Live Cell FRET Imaging.
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    Chapter 20 Wide-Field Fluorescence Lifetime Imaging with Multi-anode Detectors
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    Chapter 21 Global Analysis of FRET–FLIM Data in Live Plant Cells
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    Chapter 22 Time-Resolved Fluorescence Anisotropy Imaging
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    Chapter 23 Multimodal Fluorescence Imaging Spectroscopy
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    Chapter 24 Application of Fluorescence Correlation Spectroscopy (FCS) to Measure the Dynamics of Fluorescent Proteins in Living Cells
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    Chapter 25 Fluorescence Cross-Correlation Spectroscopy (FCCS) in Living Cells.
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    Chapter 26 Quantifying Lipid-Protein Interaction by Fluorescence Correlation Spectroscopy (FCS).
  28. Altmetric Badge
    Chapter 27 Fluorescence Spectroscopy and Microscopy
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    Chapter 28 Z-Scan Fluorescence Correlation Spectroscopy as a Tool for Diffusion Measurements in Planar Lipid Membranes
  30. Altmetric Badge
    Chapter 29 Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane.
  31. Altmetric Badge
    Chapter 30 Implementation and Application of Pulsed Interleaved Excitation for Dual-Color FCS and RICS.
  32. Altmetric Badge
    Chapter 31 Quantitative study of protein-protein interactions in live cell by dual-color fluorescence correlation spectroscopy.
  33. Altmetric Badge
    Chapter 32 Brightness Experiments
  34. Altmetric Badge
    Chapter 33 Global Analysis of Autocorrelation Functions and Photon Counting Distributions in Fluorescence Fluctuation Spectroscopy
  35. Altmetric Badge
    Chapter 34 Simulation of Autocorrelation Function and Photon Counting Distribution in Fluorescence Fluctuation Spectroscopy
  36. Altmetric Badge
    Chapter 35 Single-Molecule Fluorescence of Nucleic Acids.
  37. Altmetric Badge
    Chapter 36 Photoswitchable Fluorescent Proteins for Superresolution Fluorescence Microscopy Circumventing the Diffraction Limit of Light
Attention for Chapter 29: Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane.
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Chapter title
Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane.
Chapter number 29
Book title
Fluorescence Spectroscopy and Microscopy
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-649-8_29
Pubmed ID
Book ISBNs
978-1-62703-648-1, 978-1-62703-649-8
Authors

Paul Müller, Petra Schwille, Thomas Weidemann, Müller, Paul, Schwille, Petra, Weidemann, Thomas

Abstract

Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free-standing biomembranes. Using a confocal laser scanning microscope (CLSM), the open detection volume is repeatedly scanned through the membrane at a kHz frequency. The fluorescence photons emitted from the detection volume are continuously recorded and stored in a file. While the accessory hardware requirements for a conventional CLSM are minimal, data evaluation can pose a bottleneck. The photon events must be assigned to each scan, in which the maximum signal intensities have to be detected, binned, and aligned between the scans, in order to derive the membrane-related intensity fluctuations of one spot. Finally, this time-dependent signal must be correlated and evaluated by well-known FCS model functions. Here we provide two platform-independent, open source software tools (PyScanFCS and PyCorrFit) that allow to perform all of these steps and to establish perpendicular SFCS in its one- or two-focus as well as its single- or dual-color modality.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 22%
Other 3 17%
Student > Master 2 11%
Professor > Associate Professor 2 11%
Researcher 2 11%
Other 2 11%
Unknown 3 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 33%
Physics and Astronomy 4 22%
Biochemistry, Genetics and Molecular Biology 2 11%
Psychology 1 6%
Neuroscience 1 6%
Other 2 11%
Unknown 2 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 June 2018.
All research outputs
#17,700,887
of 22,727,570 outputs
Outputs from Methods in molecular biology
#7,175
of 13,086 outputs
Outputs of similar age
#220,723
of 305,158 outputs
Outputs of similar age from Methods in molecular biology
#261
of 594 outputs
Altmetric has tracked 22,727,570 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,086 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
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