Title |
Additive manufacturing of wet-spun polymeric scaffolds for bone tissue engineering
|
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Published in |
Biomedical Microdevices, July 2012
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DOI | 10.1007/s10544-012-9677-0 |
Pubmed ID | |
Authors |
Dario Puppi, Carlos Mota, Matteo Gazzarri, Dinuccio Dinucci, Antonio Gloria, Mairam Myrzabekova, Luigi Ambrosio, Federica Chiellini |
Abstract |
An Additive Manufacturing technique for the fabrication of three-dimensional polymeric scaffolds, based on wet-spinning of poly(ε-caprolactone) (PCL) or PCL/hydroxyapatite (HA) solutions, was developed. The processing conditions to fabricate scaffolds with a layer-by-layer approach were optimized by studying their influence on fibres morphology and alignment. Two different scaffold architectures were designed and fabricated by tuning inter-fibre distance and fibres staggering. The developed scaffolds showed good reproducibility of the internal architecture characterized by highly porous, aligned fibres with an average diameter in the range 200-250 μm. Mechanical characterization showed that the architecture and HA loading influenced the scaffold compressive modulus and strength. Cell culture experiments employing MC3T3-E1 preosteoblast cell line showed good cell adhesion, proliferation, alkaline phosphatase activity and bone mineralization on the developed scaffolds. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Portugal | 1 | <1% |
Germany | 1 | <1% |
Italy | 1 | <1% |
Australia | 1 | <1% |
United Kingdom | 1 | <1% |
Unknown | 138 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 37 | 26% |
Student > Master | 32 | 22% |
Student > Bachelor | 16 | 11% |
Researcher | 11 | 8% |
Student > Doctoral Student | 6 | 4% |
Other | 16 | 11% |
Unknown | 25 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 44 | 31% |
Materials Science | 16 | 11% |
Chemistry | 11 | 8% |
Medicine and Dentistry | 10 | 7% |
Agricultural and Biological Sciences | 8 | 6% |
Other | 23 | 16% |
Unknown | 31 | 22% |