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Use of Fluorescence Lifetime Imaging Microscopy (FLIM) as a Timer of Cell Cycle S Phase

Overview of attention for article published in PLOS ONE, December 2016
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (82nd percentile)

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22 X users

Citations

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32 Dimensions

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52 Mendeley
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Title
Use of Fluorescence Lifetime Imaging Microscopy (FLIM) as a Timer of Cell Cycle S Phase
Published in
PLOS ONE, December 2016
DOI 10.1371/journal.pone.0167385
Pubmed ID
Authors

Irina A. Okkelman, Ruslan I. Dmitriev, Tara Foley, Dmitri B. Papkovsky

Abstract

Incorporation of thymidine analogues in replicating DNA, coupled with antibody and fluorophore staining, allows analysis of cell proliferation, but is currently limited to monolayer cultures, fixed cells and end-point assays. We describe a simple microscopy imaging method for live real-time analysis of cell proliferation, S phase progression over several division cycles, effects of anti-proliferative drugs and other applications. It is based on the prominent (~ 1.7-fold) quenching of fluorescence lifetime of a common cell-permeable nuclear stain, Hoechst 33342 upon the incorporation of 5-bromo-2'-deoxyuridine (BrdU) in genomic DNA and detection by fluorescence lifetime imaging microscopy (FLIM). We show that quantitative and accurate FLIM technique allows high-content, multi-parametric dynamic analyses, far superior to the intensity-based imaging. We demonstrate its uses with monolayer cell cultures, complex 3D tissue models of tumor cell spheroids and intestinal organoids, and in physiological study with metformin treatment.

X Demographics

X Demographics

The data shown below were collected from the profiles of 22 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 52 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 21%
Student > Master 10 19%
Other 6 12%
Researcher 6 12%
Student > Postgraduate 3 6%
Other 5 10%
Unknown 11 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 23%
Agricultural and Biological Sciences 11 21%
Physics and Astronomy 3 6%
Immunology and Microbiology 2 4%
Medicine and Dentistry 2 4%
Other 7 13%
Unknown 15 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2019.
All research outputs
#2,847,302
of 24,694,993 outputs
Outputs from PLOS ONE
#34,983
of 213,628 outputs
Outputs of similar age
#54,656
of 431,062 outputs
Outputs of similar age from PLOS ONE
#685
of 3,998 outputs
Altmetric has tracked 24,694,993 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 213,628 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 431,062 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 3,998 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 82% of its contemporaries.