Title |
Mapping of Mitochondrial RNA-Protein Interactions by Digital RNase Footprinting
|
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Published in |
Cell Reports, October 2013
|
DOI | 10.1016/j.celrep.2013.09.036 |
Pubmed ID | |
Authors |
Ganqiang Liu, Timothy R. Mercer, Anne-Marie J. Shearwood, Stefan J. Siira, Moira E. Hibbs, John S. Mattick, Oliver Rackham, Aleksandra Filipovska |
Abstract |
Human mitochondrial DNA is transcribed as long polycistronic transcripts that encompass each strand of the genome and are processed subsequently into mature mRNAs, tRNAs, and rRNAs, necessitating widespread posttranscriptional regulation. Here, we establish methods for massively parallel sequencing and analyses of RNase-accessible regions of human mitochondrial RNA and thereby identify specific regions within mitochondrial transcripts that are bound by proteins. This approach provides a range of insights into the contribution of RNA-binding proteins to the regulation of mitochondrial gene expression. |
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Geographical breakdown
Country | Count | As % |
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 3 | 3% |
United Kingdom | 1 | 1% |
Unknown | 86 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 26 | 29% |
Researcher | 16 | 18% |
Professor > Associate Professor | 9 | 10% |
Professor | 7 | 8% |
Student > Master | 7 | 8% |
Other | 13 | 14% |
Unknown | 12 | 13% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 38 | 42% |
Agricultural and Biological Sciences | 25 | 28% |
Computer Science | 5 | 6% |
Medicine and Dentistry | 4 | 4% |
Engineering | 3 | 3% |
Other | 5 | 6% |
Unknown | 10 | 11% |