| Title |
A novel method for assessment of local pH in periplasmic space and of cell surface potential in yeast
|
|---|---|
| Published in |
Journal of Bioenergetics and Biomembranes, April 2017
|
| DOI | 10.1007/s10863-017-9710-3 |
| Pubmed ID | |
| Authors |
Jaromír Plášek, David Babuka, Dana Gášková, Iva Jančíková, Jakub Zahumenský, Milan Hoefer |
| Abstract |
Yeast cells exhibit a negative surface potential due to negative charges at the cell membrane surface. Consequently, local concentrations of cations at the periplasmic membrane surface may be significantly increased compared to their bulk environment. However, in cell suspensions only bulk concentrations of cations can be measured directly. Here we present a novel method enabling the assessment of local pH at the periplasmic membrane surface which can be directly related to the underlying cell surface potential. In this proof of concept study using Saccharomyces cerevisiae cells with episomally expressed pH reporter, pHluorin, intracellular acidification induced by the addition of the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP) was measured using synchronously scanned fluorescence spectroscopy (SSF). The analysis of titration curves revealed that the pH at the periplasmic surface of S. cerevisiae cells was about two units lower than the pH of bulk medium. This pH difference was significantly decreased by increasing the ionic strength of the bulk medium. The cell surface potential was estimated to amount to -130 mV. Comparable results were obtained also with another protonophore, pentachlorophenol (PCP). |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 8% |
| Unknown | 11 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 50% |
| Student > Doctoral Student | 2 | 17% |
| Professor | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Other | 1 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 50% |
| Biochemistry, Genetics and Molecular Biology | 4 | 33% |
| Chemical Engineering | 1 | 8% |
| Physics and Astronomy | 1 | 8% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 July 2021.
All research outputs
#6,824,531
of 23,867,274 outputs
Outputs from Journal of Bioenergetics and Biomembranes
#86
of 466 outputs
Outputs of similar age
#105,975
of 312,771 outputs
Outputs of similar age from Journal of Bioenergetics and Biomembranes
#2
of 6 outputs
Altmetric has tracked 23,867,274 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 466 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done well, scoring higher than 79% of its peers.
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We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.