| Title |
The endothelial cell tube formation assay on basement membrane turns 20: state of the science and the art
|
|---|---|
| Published in |
Angiogenesis, April 2009
|
| DOI | 10.1007/s10456-009-9146-4 |
| Pubmed ID | |
| Authors |
Irina Arnaoutova, Jay George, Hynda K. Kleinman, Gabriel Benton |
| Abstract |
It has been more than 20 years since it was first demonstrated that endothelial cells will rapidly form capillary-like structures in vitro when plated on top of a reconstituted basement membrane extracellular matrix (BME, Matrigel, EHS matrix, etc.). Subsequently, this morphological differentiation has been demonstrated with a variety of endothelial cells; with endothelial progenitor cells; and with transformed/immortalized endothelial cells. The differentiation process involves several steps in blood vessel formation, including cell adhesion, migration, alignment, protease secretion, and tubule formation. Because the formation of vessel structures is rapid and quantifiable, endothelial cell differentiation on basement membrane has found numerous applications in assays. Such differentiation has been used (1) to study angiogenic and antiangiogenic factors, (2) to define mechanisms and pathways involved in angiogenesis, and (3) to define endothelial cell populations. Further, the endothelial cell differentiation assay has been successfully used to study processes ranging from wound repair and reproduction to development and tumor growth. The assay is easy to perform and is the most widely used in vitro angiogenesis assay. |
Mendeley readers
The data shown below were compiled from readership statistics for 470 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | <1% |
| Canada | 2 | <1% |
| Belgium | 2 | <1% |
| Italy | 1 | <1% |
| Australia | 1 | <1% |
| Ireland | 1 | <1% |
| United Kingdom | 1 | <1% |
| Germany | 1 | <1% |
| Netherlands | 1 | <1% |
| Other | 1 | <1% |
| Unknown | 456 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 125 | 27% |
| Researcher | 77 | 16% |
| Student > Master | 60 | 13% |
| Student > Bachelor | 48 | 10% |
| Student > Doctoral Student | 32 | 7% |
| Other | 58 | 12% |
| Unknown | 70 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 127 | 27% |
| Biochemistry, Genetics and Molecular Biology | 87 | 19% |
| Medicine and Dentistry | 65 | 14% |
| Engineering | 33 | 7% |
| Chemistry | 18 | 4% |
| Other | 57 | 12% |
| Unknown | 83 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 November 2023.
All research outputs
#1,960,745
of 24,516,705 outputs
Outputs from Angiogenesis
#25
of 574 outputs
Outputs of similar age
#5,718
of 96,690 outputs
Outputs of similar age from Angiogenesis
#1
of 4 outputs
Altmetric has tracked 24,516,705 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 574 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 96,690 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 4 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them