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Mendeley readers
Attention Score in Context
| Title |
Flow Cytometric Analysis of Cell Division by Dilution of CFSE and Related Dyes
|
|---|---|
| Published in |
Current protocols in cytometry, April 2013
|
| DOI | 10.1002/0471142956.cy0911s64 |
| Pubmed ID | |
| Authors |
A Bruce Lyons, Stephen J Blake, Kathleen V Doherty |
| Abstract |
The technique described in this unit uses the intracellular fluorescent label carboxyfluorescein diacetate succinimidyl ester (CFSE) to track proliferating cells. Covalently bound CFSE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division. The technique is applicable to in vitro cell division, as well as to in vivo division of adoptively transferred cells and can resolve eight or more successive generations. CFSE is long lived, permitting analysis for several months after cell transfer, and has the same spectral characteristics as fluorescein, so monoclonal antibodies conjugated to phycoerythrin or other compatible fluorochromes may be used to immunophenotype the dividing cells. In addition, information is given on a second-generation dye, Cell Trace Violet (CTV), excited by 405-nm blue laser light. CTV is chemically related to CFSE, but allows the 488-nm line of the Argon laser to be used for other probes. |
Mendeley readers
The data shown below were compiled from readership statistics for 178 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 1% |
| United Kingdom | 1 | <1% |
| Germany | 1 | <1% |
| Korea, Republic of | 1 | <1% |
| Belgium | 1 | <1% |
| Unknown | 172 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 40 | 22% |
| Student > Bachelor | 24 | 13% |
| Student > Master | 23 | 13% |
| Researcher | 22 | 12% |
| Student > Doctoral Student | 11 | 6% |
| Other | 20 | 11% |
| Unknown | 38 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 38 | 21% |
| Biochemistry, Genetics and Molecular Biology | 31 | 17% |
| Medicine and Dentistry | 22 | 12% |
| Immunology and Microbiology | 21 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 2% |
| Other | 19 | 11% |
| Unknown | 43 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2016.
All research outputs
#8,535,472
of 25,374,917 outputs
Outputs from Current protocols in cytometry
#35
of 110 outputs
Outputs of similar age
#72,454
of 212,995 outputs
Outputs of similar age from Current protocols in cytometry
#1
of 2 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 110 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 212,995 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them