| Title |
Electrochemical transduction of DNA hybridization at modified electrodes by using an electroactive pyridoacridone intercalator
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, September 2013
|
| DOI | 10.1007/s00216-013-7314-2 |
| Pubmed ID | |
| Authors |
Laurent Bouffier, Bingquan Stuart Wang, André Roget, Thierry Livache, Martine Demeunynck, Pascal Mailley |
| Abstract |
A synthetic redox probe structurally related to natural pyridoacridones was designed and electrochemically characterised. These heterocycles behave as DNA intercalators due to their extended planar structure that promotes stacking in between nucleic acid base pairs. Electrochemical characterization by cyclic voltammetry revealed a quasi-reversible electrochemical behaviour occurring at a mild negative potential in aqueous solution. The study of the mechanism showed that the iminoquinone redox moiety acts similarly to quinone involving a two-electron reduction coupled with proton transfer. The easily accessible potential region with respect to aqueous electro-inactive window makes the pyridoacridone ring suitable for the indirect electrochemical detection of chemically unlabelled DNA. Its usefulness as electrochemical hybridization indicator was assessed on immobilised DNA and compared to doxorubicin. The voltamperometric response of the intercalator acts as an indicator of the presence of double-stranded DNA at the electrode surface and allows the selective transduction of immobilised oligonucleotide hybridization at both macro- and microscale electrodes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 31% |
| Professor | 2 | 15% |
| Student > Doctoral Student | 2 | 15% |
| Researcher | 2 | 15% |
| Professor > Associate Professor | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 2 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 6 | 46% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Materials Science | 1 | 8% |
| Chemical Engineering | 1 | 8% |
| Unknown | 3 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2013.
All research outputs
#17,285,668
of 25,374,647 outputs
Outputs from Analytical & Bioanalytical Chemistry
#5,669
of 9,619 outputs
Outputs of similar age
#132,266
of 210,951 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#38
of 80 outputs
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So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 37th percentile – i.e., 37% of its peers scored the same or lower than it.
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We're also able to compare this research output to 80 others from the same source and published within six weeks on either side of this one. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.