Title |
CFTR-dependent defect in alternatively-activated macrophages in cystic fibrosis
|
---|---|
Published in |
Journal of Cystic Fibrosis, April 2017
|
DOI | 10.1016/j.jcf.2017.03.011 |
Pubmed ID | |
Authors |
Abdullah A. Tarique, Peter D. Sly, Patrick G. Holt, Anthony Bosco, Robert S. Ware, Jayden Logan, Scott C. Bell, Claire E. Wainwright, Emmanuelle Fantino |
Abstract |
The role of the macrophages in cystic fibrosis (CF) lung disease has been poorly studied. We hypothesized that alternatively activated M2 macrophages are abnormal in CF lung disease. Blood samples were collected from adults (n=13) children (n=27) with CF on admission for acute pulmonary exacerbation and when clinically stable. Monocytes were differentiated into macrophages and polarized into classical (M1) and alternatively-activated (M2) phenotypes, function determined ex-vivo and compared with healthy controls. In the absence of functional cystic fibrosis trans-membrane conductance regulator (CFTR), either naturally in patients with CF or induced with CFTR inhibitors, monocyte-derived macrophages do not respond to IL-13/IL-4, fail to polarize into M2s associated with a post-transcriptional failure to produce and express IL-13Rα1 on the macrophage surface Polarization to the M1 phenotype was unaffected. CFTR-dependent imbalance of macrophage phenotypes and functions could contribute to the exaggerated inflammatory response seen in CF lung disease. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Australia | 1 | 17% |
United Kingdom | 1 | 17% |
Unknown | 4 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 50% |
Practitioners (doctors, other healthcare professionals) | 2 | 33% |
Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
Geographical breakdown
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Unknown | 69 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 11 | 16% |
Researcher | 9 | 13% |
Student > Ph. D. Student | 8 | 12% |
Student > Master | 7 | 10% |
Student > Doctoral Student | 5 | 7% |
Other | 14 | 20% |
Unknown | 15 | 22% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 14 | 20% |
Agricultural and Biological Sciences | 7 | 10% |
Immunology and Microbiology | 7 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
Other | 4 | 6% |
Unknown | 20 | 29% |