| Title |
The mannobiose-forming exo-mannanase involved in a new mannan catabolic pathway in Bacteroides fragilis
|
|---|---|
| Published in |
Archives of Microbiology, November 2013
|
| DOI | 10.1007/s00203-013-0938-y |
| Pubmed ID | |
| Authors |
Kazuki Kawaguchi, Takeshi Senoura, Shigeaki Ito, Toki Taira, Hiroyuki Ito, Jun Wasaki, Susumu Ito |
| Abstract |
We have proposed a new mannan catabolic pathway in Bacteroides fragilis NCTC 9343 that involves a putative mannanase ManA in glycoside hydrolase family 26 (BF0771), a mannobiose and/or sugar transporter (BF0773), mannobiose 2-epimerase (BF0774), and mannosylglucose phosphorylase (BF0772). If this hypothesis is correct, ManA has to generate mannobiose from mannans as the major end product. In this study, the BF0771 gene from the B. fragilis genome was cloned and expressed in Escherichia coli cells. The expressed protein was found to produce mannobiose exclusively from mannans and initially from manno-oligosaccharides. Production of 4-O-β-D-glucopyranosyl-D-mannose or 4-O-β-D-mannopyranosyl-D-glucose from mannans was not detectable. The results indicate that this enzyme is a novel mannobiose-forming exo-mannanase, consistent with the new microbial mannan catabolic pathway we proposed. |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 20% |
| Researcher | 5 | 13% |
| Professor > Associate Professor | 5 | 13% |
| Student > Master | 5 | 13% |
| Student > Postgraduate | 4 | 10% |
| Other | 5 | 13% |
| Unknown | 8 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 14 | 35% |
| Biochemistry, Genetics and Molecular Biology | 9 | 23% |
| Immunology and Microbiology | 3 | 8% |
| Environmental Science | 1 | 3% |
| Medicine and Dentistry | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 10 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 November 2013.
All research outputs
#5,852,820
of 22,729,647 outputs
Outputs from Archives of Microbiology
#449
of 2,765 outputs
Outputs of similar age
#52,040
of 212,425 outputs
Outputs of similar age from Archives of Microbiology
#2
of 5 outputs
Altmetric has tracked 22,729,647 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 2,765 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 212,425 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.