| Title |
Non-invasive PET Imaging of PARP1 Expression in Glioblastoma Models
|
|---|---|
| Published in |
Molecular Imaging and Biology, October 2015
|
| DOI | 10.1007/s11307-015-0904-y |
| Pubmed ID | |
| Authors |
Brandon Carney, Giuseppe Carlucci, Beatriz Salinas, Valentina Di Gialleonardo, Susanne Kossatz, Axel Vansteene, Valerie A. Longo, Alexander Bolaender, Gabriela Chiosis, Kayvan R. Keshari, Wolfgang A. Weber, Thomas Reiner |
| Abstract |
The current study presents [(18)F]PARPi as imaging agent for PARP1 expression. [(18)F]PARPi was generated by conjugating a 2H-phthalazin-1-one scaffold to 4-[(18)F]fluorobenzoic acid. Biochemical assays, optical in vivo competition, biodistribution analysis, positron emission tomography (PET)/X-ray computed tomography, and PET/magnetic resonance imaging studies were performed in subcutaneous and orthotopic mouse models of glioblastoma. [(18)F]PARPi shows suitable pharmacokinetic properties for brain tumor imaging (IC50 = 2.8 ± 1.1 nM; logPCHI = 2.15 ± 0.41; plasma-free fraction = 63.9 ± 12.6 %) and accumulates selectively in orthotopic brain tumor tissue. Tracer accumulation in subcutaneous brain tumors was 1.82 ± 0.21 %ID/g, whereas in healthy brain, the uptake was only 0.04 ± 0.01 %ID/g. [(18)F]PARPi is a selective PARP1 imaging agent that can be used to visualize glioblastoma in xenograft and orthotopic mouse models with high precision and good signal/noise ratios. It offers new opportunities to non-invasively image tumor growth and monitor interventions. |
Mendeley readers
The data shown below were compiled from readership statistics for 62 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Unknown | 61 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 26% |
| Researcher | 10 | 16% |
| Student > Master | 8 | 13% |
| Student > Bachelor | 4 | 6% |
| Student > Postgraduate | 4 | 6% |
| Other | 3 | 5% |
| Unknown | 17 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 16 | 26% |
| Medicine and Dentistry | 11 | 18% |
| Biochemistry, Genetics and Molecular Biology | 4 | 6% |
| Agricultural and Biological Sciences | 4 | 6% |
| Neuroscience | 4 | 6% |
| Other | 4 | 6% |
| Unknown | 19 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 May 2016.
All research outputs
#22,756,649
of 25,371,288 outputs
Outputs from Molecular Imaging and Biology
#687
of 837 outputs
Outputs of similar age
#251,894
of 294,211 outputs
Outputs of similar age from Molecular Imaging and Biology
#14
of 16 outputs
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