Title |
Glutaredoxin regulates vascular development by reversible glutathionylation of sirtuin 1
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Published in |
Proceedings of the National Academy of Sciences of the United States of America, November 2013
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DOI | 10.1073/pnas.1313753110 |
Pubmed ID | |
Authors |
Lars Bräutigam, Lasse Dahl Ejby Jensen, Gereon Poschmann, Staffan Nyström, Sarah Bannenberg, Kristian Dreij, Klaudia Lepka, Timour Prozorovski, Sergio J. Montano, Orhan Aktas, Per Uhlén, Kai Stühler, Yihai Cao, Arne Holmgren, Carsten Berndt |
Abstract |
Embryonic development depends on complex and precisely orchestrated signaling pathways including specific reduction/oxidation cascades. Oxidoreductases of the thioredoxin family are key players conveying redox signals through reversible posttranslational modifications of protein thiols. The importance of this protein family during embryogenesis has recently been exemplified for glutaredoxin 2, a vertebrate-specific glutathione-disulfide oxidoreductase with a critical role for embryonic brain development. Here, we discovered an essential function of glutaredoxin 2 during vascular development. Confocal microscopy and time-lapse studies based on two-photon microscopy revealed that morpholino-based knockdown of glutaredoxin 2 in zebrafish, a model organism to study vertebrate embryogenesis, resulted in a delayed and disordered blood vessel network. We were able to show that formation of a functional vascular system requires glutaredoxin 2-dependent reversible S-glutathionylation of the NAD(+)-dependent protein deacetylase sirtuin 1. Using mass spectrometry, we identified a cysteine residue in the conserved catalytic region of sirtuin 1 as target for glutaredoxin 2-specific deglutathionylation. Thereby, glutaredoxin 2-mediated redox regulation controls enzymatic activity of sirtuin 1, a mechanism we found to be conserved between zebrafish and humans. These results link S-glutathionylation to vertebrate development and successful embryonic angiogenesis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 4% |
Netherlands | 1 | 1% |
Portugal | 1 | 1% |
Japan | 1 | 1% |
Sweden | 1 | 1% |
Unknown | 78 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 22 | 26% |
Student > Master | 14 | 16% |
Student > Ph. D. Student | 13 | 15% |
Professor | 7 | 8% |
Student > Bachelor | 5 | 6% |
Other | 15 | 18% |
Unknown | 9 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 29 | 34% |
Biochemistry, Genetics and Molecular Biology | 21 | 25% |
Medicine and Dentistry | 8 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
Unspecified | 2 | 2% |
Other | 5 | 6% |
Unknown | 18 | 21% |