| Title |
PPARγ activation regulates lipid droplet formation and lactate production in rat Sertoli cells
|
|---|---|
| Published in |
Cell and Tissue Research, April 2017
|
| DOI | 10.1007/s00441-017-2615-y |
| Pubmed ID | |
| Authors |
A. Gorga, G. M. Rindone, M. Regueira, E. H. Pellizzari, M. C. Camberos, S. B. Cigorraga, M. F. Riera, M. N. Galardo, S. B. Meroni |
| Abstract |
Sertoli cells provide the structural and nutritional support for germ cell development; they actively metabolize glucose and convert it to lactate, which is an important source of energy for germ cells. Furthermore, Sertoli cells can oxidize fatty acids, a metabolic process that is assumed to fulfill their own energy requirements. Fatty acids are stored as triacylglycerides within lipid droplets. The regulation of fatty acid storage in conjunction with the regulation of lactate production may thus be relevant to seminiferous tubule physiology. Our aim is to evaluate a possible means of regulation by the PPARγ activation of lipid droplet formation and lactate production. Sertoli cell cultures obtained from 20-day-old rats were incubated with Rosiglitazone (10 μM), a PPARγ activator, for various periods of time (6, 12, 24 and 48 h). Increased triacylglycerides levels and lipid droplet content were observed, accompanied by a rise in the expression of genes for proteins involved in fatty acid storage, such as the fatty acid transporter Cd36, glycerol-3-phosphate-acyltransferases 1 and 3, diacylglycerol acyltransferase 1 and perilipins 1, 2 and 3, all proteins that participate in lipid droplet formation and stabilization. However, PPARγ activation increased lactate production, accompanied by an augmentation in glucose uptake and Glut2 expression. These results taken together suggest that PPARγ activation in Sertoli cells participates in the regulation of lipid storage and lactate production thereby ensuring simultaneously the energetic metabolism for the Sertoli and germ cells. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 32 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 38% |
| Student > Ph. D. Student | 7 | 22% |
| Student > Master | 3 | 9% |
| Professor | 1 | 3% |
| Librarian | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 6 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 31% |
| Agricultural and Biological Sciences | 6 | 19% |
| Medicine and Dentistry | 3 | 9% |
| Economics, Econometrics and Finance | 1 | 3% |
| Business, Management and Accounting | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 9 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 April 2017.
All research outputs
#21,180,380
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#2,002
of 2,279 outputs
Outputs of similar age
#271,896
of 311,723 outputs
Outputs of similar age from Cell and Tissue Research
#29
of 32 outputs
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