Title |
Emerging potential of gene silencing approaches targeting anti-chondrogenic factors for cell-based cartilage repair
|
---|---|
Published in |
Cellular and Molecular Life Sciences, April 2017
|
DOI | 10.1007/s00018-017-2531-z |
Pubmed ID | |
Authors |
Andrea Lolli, Letizia Penolazzi, Roberto Narcisi, Gerjo J. V. M. van Osch, Roberta Piva |
Abstract |
The field of cartilage repair has exponentially been growing over the past decade. Here, we discuss the possibility to achieve satisfactory regeneration of articular cartilage by means of human mesenchymal stem cells (hMSCs) depleted of anti-chondrogenic factors and implanted in the site of injury. Different types of molecules including transcription factors, transcriptional co-regulators, secreted proteins, and microRNAs have recently been identified as negative modulators of chondroprogenitor differentiation and chondrocyte function. We review the current knowledge about these molecules as potential targets for gene knockdown strategies using RNA interference (RNAi) tools that allow the specific suppression of gene function. The critical issues regarding the optimization of the gene silencing approach as well as the delivery strategies are discussed. We anticipate that further development of these techniques will lead to the generation of implantable hMSCs with enhanced potential to regenerate articular cartilage damaged by injury, disease, or aging. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Ireland | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 25 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 5 | 20% |
Researcher | 4 | 16% |
Student > Bachelor | 3 | 12% |
Student > Ph. D. Student | 3 | 12% |
Student > Doctoral Student | 2 | 8% |
Other | 3 | 12% |
Unknown | 5 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 8 | 32% |
Biochemistry, Genetics and Molecular Biology | 6 | 24% |
Agricultural and Biological Sciences | 4 | 16% |
Nursing and Health Professions | 1 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 1 | 4% |
Unknown | 4 | 16% |