Title |
Glycolytic control of vacuolar-type ATPase activity: A mechanism to regulate influenza viral infection
|
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Published in |
Virology, July 2013
|
DOI | 10.1016/j.virol.2013.06.026 |
Pubmed ID | |
Authors |
Hinissan P. Kohio, Amy L. Adamson |
Abstract |
As new influenza virus strains emerge, finding new mechanisms to control infection is imperative. In this study, we found that we could control influenza infection of mammalian cells by altering the level of glucose given to cells. Higher glucose concentrations induced a dose-specific increase in influenza infection. Linking influenza virus infection with glycolysis, we found that viral replication was significantly reduced after cells were treated with glycolytic inhibitors. Addition of extracellular ATP after glycolytic inhibition restored influenza infection. We also determined that higher levels of glucose promoted the assembly of the vacuolar-type ATPase within cells, and increased vacuolar-type ATPase proton-transport activity. The increase of viral infection via high glucose levels could be reversed by inhibition of the proton pump, linking glucose metabolism, vacuolar-type ATPase activity, and influenza viral infection. Taken together, we propose that altering glucose metabolism may be a potential new approach to inhibit influenza viral infection. |
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Country | Count | As % |
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United Kingdom | 40 | 7% |
Spain | 17 | 3% |
Canada | 14 | 3% |
Australia | 14 | 3% |
Brazil | 7 | 1% |
South Africa | 7 | 1% |
India | 7 | 1% |
Chile | 4 | <1% |
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Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 54 | 10% |
Scientists | 23 | 4% |
Science communicators (journalists, bloggers, editors) | 2 | <1% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Mexico | 1 | <1% |
United States | 1 | <1% |
Unknown | 111 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 17 | 15% |
Student > Bachelor | 14 | 12% |
Student > Master | 8 | 7% |
Other | 8 | 7% |
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Unknown | 23 | 20% |
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Nursing and Health Professions | 4 | 4% |
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