Title |
PARP1 restricts Epstein Barr Virus lytic reactivation by binding the BZLF1 promoter
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Published in |
Virology, April 2017
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DOI | 10.1016/j.virol.2017.04.006 |
Pubmed ID | |
Authors |
Lena N Lupey-Green, Stephanie A Moquin, Kayla A Martin, Shane M McDevitt, Michael Hulse, Lisa B Caruso, Richard T Pomerantz, Jj L Miranda, Italo Tempera |
Abstract |
The Epstein Barr virus (EBV) genome persists in infected host cells as a chromatinized episome and is subject to chromatin-mediated regulation. Binding of the host insulator protein CTCF to the EBV genome has an established role in maintaining viral latency type, and in other herpesviruses, loss of CTCF binding at specific regions correlates with viral reactivation. Here, we demonstrate that binding of PARP1, an important cofactor of CTCF, at the BZLF1 lytic switch promoter restricts EBV reactivation. Knockdown of PARP1 in the Akata-EBV cell line significantly increases viral copy number and lytic protein expression. Interestingly, CTCF knockdown has no effect on viral reactivation, and CTCF binding across the EBV genome is largely unchanged following reactivation. Moreover, EBV reactivation attenuates PARP activity, and Zta expression alone is sufficient to decrease PARP activity. Here we demonstrate a restrictive function of PARP1 in EBV lytic reactivation. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 23% |
Student > Doctoral Student | 4 | 15% |
Researcher | 3 | 12% |
Student > Master | 3 | 12% |
Student > Bachelor | 2 | 8% |
Other | 1 | 4% |
Unknown | 7 | 27% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 5 | 19% |
Biochemistry, Genetics and Molecular Biology | 4 | 15% |
Agricultural and Biological Sciences | 2 | 8% |
Chemistry | 2 | 8% |
Immunology and Microbiology | 2 | 8% |
Other | 4 | 15% |
Unknown | 7 | 27% |