| Title |
Precision knockdown of EGFR gene expression using radio frequency electromagnetic energy
|
|---|---|
| Published in |
Journal of Neuro-Oncology, April 2017
|
| DOI | 10.1007/s11060-017-2440-x |
| Pubmed ID | |
| Authors |
Ilya V. Ulasov, Haidn Foster, Mike Butters, Jae-Geun Yoon, Tomoko Ozawa, Theodore Nicolaides, Xavier Figueroa, Parvinder Hothi, Michael Prados, John Butters, Charles Cobbs |
| Abstract |
Electromagnetic fields (EMF) in the radio frequency energy (RFE) range can affect cells at the molecular level. Here we report a technology that can record the specific RFE signal of a given molecule, in this case the siRNA of epidermal growth factor receptor (EGFR). We demonstrate that cells exposed to this EGFR siRNA RFE signal have a 30-70% reduction of EGFR mRNA expression and ~60% reduction in EGFR protein expression vs. control treated cells. Specificity for EGFR siRNA effect was confirmed via RNA microarray and antibody dot blot array. The EGFR siRNA RFE decreased cell viability, as measured by Calcein-AM measures, LDH release and Caspase 3 cleavage, and increased orthotopic xenograft survival. The outcomes of this study demonstrate that an RFE signal can induce a specific siRNA-like effect on cells. This technology opens vast possibilities of targeting a broader range of molecules with applications in medicine, agriculture and other areas. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 16 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 38% |
| Student > Ph. D. Student | 4 | 25% |
| Unspecified | 1 | 6% |
| Student > Postgraduate | 1 | 6% |
| Unknown | 4 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 19% |
| Medicine and Dentistry | 2 | 13% |
| Engineering | 2 | 13% |
| Agricultural and Biological Sciences | 2 | 13% |
| Unspecified | 1 | 6% |
| Other | 2 | 13% |
| Unknown | 4 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 40. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 April 2023.
All research outputs
#914,309
of 23,760,369 outputs
Outputs from Journal of Neuro-Oncology
#28
of 3,058 outputs
Outputs of similar age
#19,720
of 310,901 outputs
Outputs of similar age from Journal of Neuro-Oncology
#1
of 101 outputs
Altmetric has tracked 23,760,369 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,058 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 310,901 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 93% of its contemporaries.
We're also able to compare this research output to 101 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.