| Title |
Vitamin B12 Deficiency Stimulates Osteoclastogenesis via Increased Homocysteine and Methylmalonic Acid
|
|---|---|
| Published in |
Calcified Tissue International, April 2009
|
| DOI | 10.1007/s00223-009-9244-8 |
| Pubmed ID | |
| Authors |
Bart L. T. Vaes, Carolien Lute, Henk J. Blom, Nathalie Bravenboer, Teun J. de Vries, Vincent Everts, Rosalie A. Dhonukshe-Rutten, Michael Müller, Lisette C. P. G. M. de Groot, Wilma T. Steegenga |
| Abstract |
The risk of nutrient deficiencies increases with age in our modern Western society, and vitamin B(12) deficiency is especially prevalent in the elderly and causes increased homocysteine (Hcy) and methylmalonic acid (MMA) levels. These three factors have been recognized as risk factors for reduced bone mineral density and increased fracture risk, though mechanistic evidence is still lacking. In the present study, we investigated the influence of B(12), Hcy, and MMA on differentiation and activity of bone cells. B(12) deficiency did not affect the onset of osteoblast differentiation, maturation, matrix mineralization, or adipocyte differentiation from human mesenchymal stem cells (hMSCs). B(12) deficiency caused an increase in the secretion of Hcy and MMA into the culture medium by osteoblasts, but Hcy and MMA appeared to have no effect on hMSC osteoblast differentiation. We further studied the effect of B(12), Hcy, and MMA on the formation of multinucleated tartrate-resistant acid phosphatase-positive osteoclasts from mouse bone marrow. We observed that B(12) did not show an effect on osteoclastogenesis. However, Hcy as well as MMA were found to induce osteoclastogenesis in a dose-dependent manner. On the basis of these results, we conclude that B(12) deficiency may lead to decreased bone mass by increased osteoclast formation due to increased MMA and Hcy levels. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 2% |
| Unknown | 60 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 15% |
| Student > Ph. D. Student | 6 | 10% |
| Professor | 6 | 10% |
| Student > Bachelor | 6 | 10% |
| Student > Postgraduate | 5 | 8% |
| Other | 11 | 18% |
| Unknown | 18 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 17 | 28% |
| Biochemistry, Genetics and Molecular Biology | 6 | 10% |
| Agricultural and Biological Sciences | 5 | 8% |
| Immunology and Microbiology | 2 | 3% |
| Engineering | 2 | 3% |
| Other | 9 | 15% |
| Unknown | 20 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 December 2013.
All research outputs
#15,288,160
of 22,736,112 outputs
Outputs from Calcified Tissue International
#1,335
of 1,756 outputs
Outputs of similar age
#78,893
of 93,475 outputs
Outputs of similar age from Calcified Tissue International
#4
of 5 outputs
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