| Title |
In toto differentiation of human amniotic membrane towards the Schwann cell lineage
|
|---|---|
| Published in |
Cell and Tissue Banking, October 2013
|
| DOI | 10.1007/s10561-013-9401-1 |
| Pubmed ID | |
| Authors |
Asmita Banerjee, Sylvia Nürnberger, Simone Hennerbichler, Sabrina Riedl, Christina M. A. P. Schuh, Ara Hacobian, Andreas Teuschl, Johann Eibl, Heinz Redl, Susanne Wolbank |
| Abstract |
Human amniotic membrane (hAM) is a tissue containing cells with proven stem cell properties. In its decellularized form it has been successfully applied as nerve conduit biomaterial to improve peripheral nerve regeneration in injury models. We hypothesize that viable hAM without prior cell isolation can be differentiated towards the Schwann cell lineage to generate a possible alternative to commonly applied tissue engineering materials for nerve regeneration. For in vitro Schwann cell differentiation, biopsies of hAM of 8 mm diameter were incubated with a sequential order of neuronal induction and growth factors for 21 days and characterized for cellular viability and the typical glial markers glial fibrillary acidic protein (GFAP), S100β, p75 and neurotrophic tyrosine kinase receptor (NTRK) using immunohistology. The secretion of the neurotrophic factors brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) was quantified by ELISA. The hAM maintained high viability, especially under differentiation conditions (90.2 % ± 41.6 day 14; 80.0 % ± 44.5 day 21 compared to day 0). Both, BDNF and GDNF secretion was up-regulated upon differentiation. The fresh membrane stained positive for GFAP and p75 and NTRK, which was strongly increased after culture in differentiation conditions. Especially the epithelial layer within the membrane exhibited a change in morphology upon differentiation forming a multi-layered epithelium with intense accumulations of the marker proteins. However, S100β was expressed at equal levels and equal distribution in fresh and cultured hAM conditions. Viable hAM may be a promising alternative to present formulations used for peripheral nerve regeneration. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 2% |
| Austria | 1 | 2% |
| Unknown | 41 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 16% |
| Student > Doctoral Student | 6 | 14% |
| Student > Bachelor | 6 | 14% |
| Student > Master | 6 | 14% |
| Researcher | 4 | 9% |
| Other | 4 | 9% |
| Unknown | 10 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 8 | 19% |
| Agricultural and Biological Sciences | 8 | 19% |
| Biochemistry, Genetics and Molecular Biology | 4 | 9% |
| Engineering | 2 | 5% |
| Chemistry | 2 | 5% |
| Other | 4 | 9% |
| Unknown | 15 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 December 2013.
All research outputs
#20,215,721
of 22,738,543 outputs
Outputs from Cell and Tissue Banking
#217
of 287 outputs
Outputs of similar age
#185,249
of 212,698 outputs
Outputs of similar age from Cell and Tissue Banking
#3
of 5 outputs
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