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Mendeley readers
Attention Score in Context
| Title |
Excited state dynamics of the photoconvertible fluorescent protein Kaede revealed by ultrafast spectroscopy
|
|---|---|
| Published in |
Photochemical & Photobiological Sciences, June 2014
|
| DOI | 10.1039/c3pp50335f |
| Pubmed ID | |
| Authors |
Eduard Fron, Michel Sliwa, Virgile Adam, Jan Michiels, Susana Rocha, Peter Dedecker, Johan Hofkens, Hideaki Mizuno |
| Abstract |
The ultrafast excited state dynamics of the fluorescent protein Kaede has been investigated by employing time resolved fluorescence and transient absorption. Upon irradiation of its neutral state, the protein undergoes an efficient conversion to a state that fluoresces at longer wavelengths. The molecular basis of the photoconversion involves an expansion of the chromophore π-conjugation by formal β-elimination but details of the reaction pathway remain subject to debate. Based on the kinetics observed in experiments on the protein sample in both H2O and D2O buffers, we suggest that a light-initiated cleavage mechanism (20 ps) could take place, forming the neutral red state in which the red chromophore resides. Excitation of the neutral red form results in the formation of the red anionic species via two Förster resonance energy transfer (FRET) channels. FRET between red neutral and red anionic forms occurs within the tetramer with time constants of 13.4 ps and 210 ps. In contrast to literature proposals no ESPT was observed. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Hungary | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 3% |
| Argentina | 1 | 3% |
| Belgium | 1 | 3% |
| Russia | 1 | 3% |
| United States | 1 | 3% |
| Unknown | 32 | 86% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 32% |
| Student > Ph. D. Student | 6 | 16% |
| Student > Master | 4 | 11% |
| Student > Postgraduate | 3 | 8% |
| Professor | 2 | 5% |
| Other | 6 | 16% |
| Unknown | 4 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 18 | 49% |
| Agricultural and Biological Sciences | 9 | 24% |
| Physics and Astronomy | 2 | 5% |
| Environmental Science | 1 | 3% |
| Earth and Planetary Sciences | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 5 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 December 2013.
All research outputs
#19,017,658
of 23,577,654 outputs
Outputs from Photochemical & Photobiological Sciences
#1,074
of 1,591 outputs
Outputs of similar age
#165,268
of 228,640 outputs
Outputs of similar age from Photochemical & Photobiological Sciences
#12
of 15 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,591 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 5th percentile – i.e., 5% of its peers scored the same or lower than it.
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