| Title |
Development of a recombinase polymerase amplification assay for the diagnosis of banana bunchy top virus in different banana cultivars
|
|---|---|
| Published in |
Archives of Virology, May 2017
|
| DOI | 10.1007/s00705-017-3399-9 |
| Pubmed ID | |
| Authors |
Reetika Kapoor, Nishant Srivastava, Shailender Kumar, R. K. Saritha, Susheel Kumar Sharma, Rakesh Kumar Jain, Virendra Kumar Baranwal |
| Abstract |
Recombinase polymerase amplification (RPA) is a rapid, isothermal amplification method with high specificity and sensitivity. In this study, an assay was developed and evaluated for the detection of banana bunchy top virus (BBTV) in infected banana plants. Three oligonucleotide primer pairs were designed from the replicase initiator protein gene sequences of BBTV to function both in RPA as well as in polymerase chain reaction (PCR). A total of 133 symptomatic as well as asymptomatic banana leaf samples from various cultivars were collected from the different regions of India and evaluated for BBTV infection using the RPA assay. BBTV was efficiently detected using crude leaf sap in RPA and the results obtained were consistent with PCR-based detection using purified DNA as template. To our knowledge, this is the first report of reliable diagnosis of BBTV infection by RPA using crude leaf sap as a template. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 66 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 9 | 14% |
| Researcher | 7 | 11% |
| Student > Ph. D. Student | 7 | 11% |
| Unspecified | 5 | 8% |
| Student > Postgraduate | 3 | 5% |
| Other | 7 | 11% |
| Unknown | 28 | 42% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 17 | 26% |
| Unspecified | 5 | 8% |
| Engineering | 4 | 6% |
| Biochemistry, Genetics and Molecular Biology | 3 | 5% |
| Immunology and Microbiology | 2 | 3% |
| Other | 3 | 5% |
| Unknown | 32 | 48% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 December 2017.
All research outputs
#20,420,242
of 22,971,207 outputs
Outputs from Archives of Virology
#3,411
of 4,206 outputs
Outputs of similar age
#270,050
of 310,149 outputs
Outputs of similar age from Archives of Virology
#58
of 88 outputs
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