| Title |
Efficacy of a Broad Host Range Lytic Bacteriophage Against E. coli Adhered to Urothelium
|
|---|---|
| Published in |
Current Microbiology, December 2010
|
| DOI | 10.1007/s00284-010-9834-8 |
| Pubmed ID | |
| Authors |
Sanna Sillankorva, Dulce Oliveira, Alexandra Moura, Mariana Henriques, Alberta Faustino, Ana Nicolau, Joana Azeredo |
| Abstract |
Persistent urinary tract infections (UTI) are often caused by E. coli adhered to urothelium. This type of cells is generally recognized as very tolerant to antibiotics which renders difficult the treatment of chronic UTI. This study investigates the use of lytic bacteriophages as alternative antimicrobial agents, particularly the interaction of phages with E. coli adhered to urothelium and specifically determines their efficiency against this type of cells. The bacterial adhesion to urothelium was performed varying the bacterial cell concentrations and the period and conditions (static, shaken) of adhesion. Three collection bacteriophages (T1, T4, and phiX174 like phages) were tested against clinical E. coli isolates and only one was selected for further infection experiments. Based on the lytic spectrum against clinical isolates and its ability to infect the highest number of antibiotic resistant strains, the T1-like bacteriophage was selected. This bacteriophage caused nearly a 45% reduction of the bacterial population after 2 h of treatment. This study provides evidence that bacteriophages are effective in controlling suspended and adhered cells and therefore can be a viable alternative to antibiotics to control urothelium- adhered bacteria. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Portugal | 1 | 1% |
| France | 1 | 1% |
| Australia | 1 | 1% |
| Brazil | 1 | 1% |
| India | 1 | 1% |
| Unknown | 76 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 16 | 20% |
| Student > Ph. D. Student | 15 | 19% |
| Student > Bachelor | 9 | 11% |
| Student > Master | 9 | 11% |
| Student > Doctoral Student | 4 | 5% |
| Other | 9 | 11% |
| Unknown | 19 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 21 | 26% |
| Immunology and Microbiology | 14 | 17% |
| Biochemistry, Genetics and Molecular Biology | 10 | 12% |
| Medicine and Dentistry | 6 | 7% |
| Engineering | 2 | 2% |
| Other | 4 | 5% |
| Unknown | 24 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 January 2014.
All research outputs
#15,289,831
of 22,738,543 outputs
Outputs from Current Microbiology
#1,405
of 2,404 outputs
Outputs of similar age
#140,543
of 180,470 outputs
Outputs of similar age from Current Microbiology
#14
of 18 outputs
Altmetric has tracked 22,738,543 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,404 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 35th percentile – i.e., 35% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,470 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.