| Title |
Investigating the Structural Compaction of Biomolecules Upon Transition to the Gas-Phase Using ESI-TWIMS-MS
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, May 2017
|
| DOI | 10.1007/s13361-017-1689-9 |
| Pubmed ID | |
| Authors |
Paul W. A. Devine, Henry C. Fisher, Antonio N. Calabrese, Fiona Whelan, Daniel R. Higazi, Jennifer R. Potts, David C. Lowe, Sheena E. Radford, Alison E. Ashcroft |
| Abstract |
Collision cross-section (CCS) measurements obtained from ion mobility spectrometry-mass spectrometry (IMS-MS) analyses often provide useful information concerning a protein's size and shape and can be complemented by modeling procedures. However, there have been some concerns about the extent to which certain proteins maintain a native-like conformation during the gas-phase analysis, especially proteins with dynamic or extended regions. Here we have measured the CCSs of a range of biomolecules including non-globular proteins and RNAs of different sequence, size, and stability. Using traveling wave IMS-MS, we show that for the proteins studied, the measured CCS deviates significantly from predicted CCS values based upon currently available structures. The results presented indicate that these proteins collapse to different extents varying on their elongated structures upon transition into the gas-phase. Comparing two RNAs of similar mass but different solution structures, we show that these biomolecules may also be susceptible to gas-phase compaction. Together, the results suggest that caution is needed when predicting structural models based on CCS data for RNAs as well as proteins with non-globular folds. Graphical Abstract ᅟ. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 33% |
| Unknown | 4 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 50% |
| Scientists | 2 | 33% |
| Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Unknown | 64 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 28% |
| Researcher | 10 | 15% |
| Student > Master | 8 | 12% |
| Student > Bachelor | 4 | 6% |
| Professor | 3 | 5% |
| Other | 5 | 8% |
| Unknown | 17 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 28 | 43% |
| Biochemistry, Genetics and Molecular Biology | 10 | 15% |
| Agricultural and Biological Sciences | 4 | 6% |
| Physics and Astronomy | 3 | 5% |
| Engineering | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 19 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 August 2017.
All research outputs
#14,300,634
of 25,411,814 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#2,095
of 3,842 outputs
Outputs of similar age
#154,680
of 324,841 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#18
of 72 outputs
Altmetric has tracked 25,411,814 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,842 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 324,841 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.
We're also able to compare this research output to 72 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 75% of its contemporaries.