| Title |
Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells
|
|---|---|
| Published in |
Cell and Tissue Research, January 2014
|
| DOI | 10.1007/s00441-013-1770-z |
| Pubmed ID | |
| Authors |
Wei Lu, Kun Ji, Jennifer Kirkham, Yu Yan, Aldo R. Boccaccini, Margaret Kellett, Yan Jin, Xuebin B. Yang |
| Abstract |
Translational research in bone tissue engineering is essential for "bench to bedside" patient benefit. However, the ideal combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study is to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 wt% and 40 wt% of Bioglass particles with human adipose-derived stem cells (ADSCs) in vitro and in vivo. Live/dead fluorescent markers, confocal microscopy and scanning electron microscopy showed that PDLLA/Bioglass porous scaffolds supported ADSC attachment, growth and osteogenic differentiation, as confirmed by enhanced alkaline phosphatase (ALP) activity. Higher Bioglass content of the PDLLA foams increased ALP activity compared with the PDLLA only group. Extracellular matrix deposition after 8 weeks in the in vitro cultures was evident by Alcian blue/Sirius red staining. In vivo bone formation was assessed by using scaffold/ADSC constructs in diffusion chambers transplanted intraperitoneally into nude mice and recovered after 8 weeks. Histological and immunohistochemical assays indicated significant new bone formation in the 40 wt% and 5 wt% Bioglass constructs compared with the PDLLA only group. Thus, the combination of a well-developed biodegradable bioactive porous PDLLA/Bioglass composite scaffold with a high-potential stem cell source (human ADSCs) could be a promising approach for bone regeneration in a clinical setting. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 33% |
| United States | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 92 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 1 | 1% |
| Unknown | 91 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 20% |
| Student > Master | 18 | 20% |
| Researcher | 13 | 14% |
| Student > Bachelor | 9 | 10% |
| Student > Doctoral Student | 6 | 7% |
| Other | 12 | 13% |
| Unknown | 16 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 15 | 16% |
| Agricultural and Biological Sciences | 11 | 12% |
| Materials Science | 11 | 12% |
| Biochemistry, Genetics and Molecular Biology | 10 | 11% |
| Engineering | 10 | 11% |
| Other | 17 | 18% |
| Unknown | 18 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2014.
All research outputs
#14,398,884
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#1,325
of 2,279 outputs
Outputs of similar age
#172,609
of 310,350 outputs
Outputs of similar age from Cell and Tissue Research
#18
of 23 outputs
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So far Altmetric has tracked 2,279 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
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