| Chapter title |
Analysis of variation in chloroplast DNA sequences.
|
|---|---|
| Chapter number | 5 |
| Book title |
Molecular Plant Taxonomy
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-62703-767-9_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-766-2, 978-1-62703-767-9
|
| Authors |
Berthold Heinze, Agnieszka Koziel-Monte, Daniela Jahn, Heinze B, Koziel-Monte A, Jahn D, Heinze, Berthold, Koziel-Monte, Agnieszka, Jahn, Daniela |
| Abstract |
This chapter introduces and reviews methods for analyzing variation in chloroplast DNA, mainly by polymerase chain reaction (PCR) and subsequent revelation of polymorphisms. Sources for chloroplast primers are discussed, as well as methods such as Sanger sequencing, PCR followed by restriction fragment length polymorphism (RFLP), gel electrophoresis, fragment analysis on automated DNA sequencers, denaturing high-performance liquid chromatography (dHPLC), and next-generation sequencing (NGS). A special section deals with peculiarities of chloroplast DNA variation, such as tandem repeats and mini- and microsatellites. |
X Demographics
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|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 8% |
| Unknown | 11 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 25% |
| Student > Postgraduate | 2 | 17% |
| Student > Ph. D. Student | 2 | 17% |
| Student > Doctoral Student | 1 | 8% |
| Student > Bachelor | 1 | 8% |
| Other | 2 | 17% |
| Unknown | 1 | 8% |
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|---|---|---|
| Agricultural and Biological Sciences | 8 | 67% |
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| Unknown | 2 | 17% |