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Antibiofilm activity and mode of action of DMSO alone and its combination with afatinib against Gram-negative pathogens

Overview of attention for article published in Folia Microbiologica, May 2017
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Title
Antibiofilm activity and mode of action of DMSO alone and its combination with afatinib against Gram-negative pathogens
Published in
Folia Microbiologica, May 2017
DOI 10.1007/s12223-017-0532-9
Pubmed ID
Authors

Mohd Fakharul Zaman Raja Yahya, Zazali Alias, Saiful Anuar Karsani

Abstract

Biofilms are complex microbial communities that tend to attach to either biotic or abiotic surface. Enclosed in a self-produced extracellular polymeric substance (EPS) matrix, the biofilms often cause persistent infections. The objective of this study was to investigate the antibiofilm activity of dimethyl sulfoxide (DMSO) and afatinib against Gram-negative pathogens. Test microorganisms used in this study were Escherichia coli ATCC 1299, Pseudomonas aeruginosa ATCC 10145, and Salmonella typhimurium ATCC 14028. Biofilms were developed in 96-well microplate at 37°C for 24 h. Following removal of non-adherent cells, analysis of biofilm viability, biofilm biomass, and extracellular polymeric substances (EPS) matrix were performed using resazurin assay, crystal violet assay, and attenuated total reflectance fourier transform infrared (ATR-FTIR) spectroscopy, respectively. Bradford protein assay was conducted to determine the total amount of EPS proteins. The results demonstrated that both 32% DMSO alone and its combination with 3.2 μg/mL afatinib were effective in killing biofilm cells and reducing biofilm biomass. IR spectral variations of EPS matrix of biofilms in the range between 1700 and 900 cm(-1) were also observed. Reduction in EPS proteins verified the chemical modifications of EPS matrix. In conclusion, 32% DMSO alone and its combination with 3.2 μg/mL afatinib showed remarkable antibiofilm activities against Gram-negative pathogens. It was suggested that the biofilm inhibition was mediated by the chemical modification of EPS matrix.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 36 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 25%
Student > Doctoral Student 3 8%
Student > Master 3 8%
Researcher 3 8%
Unspecified 2 6%
Other 5 14%
Unknown 11 31%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 5 14%
Biochemistry, Genetics and Molecular Biology 5 14%
Unspecified 2 6%
Medicine and Dentistry 2 6%
Immunology and Microbiology 2 6%
Other 5 14%
Unknown 15 42%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 May 2017.
All research outputs
#14,349,470
of 22,977,819 outputs
Outputs from Folia Microbiologica
#410
of 748 outputs
Outputs of similar age
#175,321
of 313,673 outputs
Outputs of similar age from Folia Microbiologica
#1
of 10 outputs
Altmetric has tracked 22,977,819 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 748 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,673 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 41st percentile – i.e., 41% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them