Chapter title |
Next-Generation Sequencing for MicroRNA Expression Profile
|
---|---|
Chapter number | 12 |
Book title |
Bioinformatics in MicroRNA Research
|
Published in |
Methods in molecular biology, May 2017
|
DOI | 10.1007/978-1-4939-7046-9_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7044-5, 978-1-4939-7046-9
|
Authors |
Hu, Yue, Lan, Wenjun, Miller, Daniel, Yue Hu, Wenjun Lan Ph.D., Daniel Miller, Wenjun Lan |
Editors |
Jingshan Huang, Glen M. Borchert, Dejing Dou, Jun (Luke) Huan, Wenjun Lan, Ming Tan, Bin Wu |
Abstract |
Sequencing technologies have made considerable advancements. From the Sanger sequencing method to the next-generation sequencing (NGS) methods, and from the NGS methods to the third-generation sequencing methods, we can see the development thread of the sequencing technology. Currently, NGS is the main contender in the sequencing market. NGS technologies provide an opportunity to research the microRNA (miRNA) expression profiles in detail. The NGS platforms have their own special characteristics, but share some main ideas. DNA sequencing via NGS is fundamental for RNA sequencing and miRNA sequencing. MiRNA sequencing has special characteristics. The pipeline of miRNA sequencing by NGS is explained in detail from the wet experiment to the dry experiment. |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 71 | 100% |
Demographic breakdown
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Student > Bachelor | 9 | 13% |
Student > Ph. D. Student | 8 | 11% |
Researcher | 7 | 10% |
Student > Master | 5 | 7% |
Student > Doctoral Student | 4 | 6% |
Other | 6 | 8% |
Unknown | 32 | 45% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 17 | 24% |
Agricultural and Biological Sciences | 3 | 4% |
Medicine and Dentistry | 3 | 4% |
Computer Science | 2 | 3% |
Veterinary Science and Veterinary Medicine | 2 | 3% |
Other | 11 | 15% |
Unknown | 33 | 46% |