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X Demographics
Mendeley readers
Attention Score in Context
| Title |
No evidence of genome editing activity from Natronobacterium gregoryi Argonaute (NgAgo) in human cells
|
|---|---|
| Published in |
PLOS ONE, May 2017
|
| DOI | 10.1371/journal.pone.0177444 |
| Pubmed ID | |
| Authors |
Parisa Javidi-Parsijani, Guoguang Niu, Meghan Davis, Pin Lu, Anthony Atala, Baisong Lu |
| Abstract |
The argonaute protein from the thermophilic bacterium Thermus thermophilus shows DNA-guided DNA interfering activity at high temperatures, complicating its application in mammalian cells. A recent work reported that the argonaute protein from Natronobacterium gregoryi (NgAgo) had DNA-guided genome editing activity in mammalian cells. We compared the genome editing activities of NgAgo and Staphylococcus aureus Cas9 (SaCas9) in human HEK293T cells side by side. EGFP reporter assays and DNA sequencing consistently revealed high genome editing activity from SaCas9. However, these assays did not demonstrate genome editing activity by NgAgo. We confirmed that the conditions allowed simultaneous transfection of the NgAgo expressing plasmid DNA and DNA guides, as well as heterologous expression of NgAgo in the HEK293T cells. Our data show that NgAgo is not a robust genome editing tool, although it may have such activity under other conditions. |
X Demographics
The data shown below were collected from the profiles of 25 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 7 | 28% |
| United States | 4 | 16% |
| Australia | 2 | 8% |
| Belgium | 1 | 4% |
| Argentina | 1 | 4% |
| Peru | 1 | 4% |
| Unknown | 9 | 36% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 14 | 56% |
| Scientists | 9 | 36% |
| Practitioners (doctors, other healthcare professionals) | 1 | 4% |
| Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 23% |
| Student > Master | 5 | 19% |
| Researcher | 4 | 15% |
| Student > Bachelor | 2 | 8% |
| Professor | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 15 | 58% |
| Agricultural and Biological Sciences | 2 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Arts and Humanities | 1 | 4% |
| Social Sciences | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 5 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 45. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 December 2020.
All research outputs
#876,999
of 24,552,012 outputs
Outputs from PLOS ONE
#11,659
of 212,005 outputs
Outputs of similar age
#18,159
of 315,286 outputs
Outputs of similar age from PLOS ONE
#263
of 4,404 outputs
Altmetric has tracked 24,552,012 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 212,005 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.6. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,286 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 4,404 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.