| Title |
Normal and Cancerous Tissues Release Extrachromosomal Circular DNA (eccDNA) into the Circulation
|
|---|---|
| Published in |
Molecular Cancer Research, August 2017
|
| DOI | 10.1158/1541-7786.mcr-17-0095 |
| Pubmed ID | |
| Authors |
Pankaj Kumar, Laura W Dillon, Yoshiyuki Shibata, Amir A Jazaeri, David R Jones, Anindya Dutta |
| Abstract |
Cell-free circulating linear DNA is being explored for non-invasive diagnosis and management of tumors and fetuses, the so-called liquid biopsy. Previously, we observed the presence of small extrachromosomal circular DNA (eccDNA), called microDNA, in the nuclei of mammalian tissues and cell lines. Now, we demonstrate that cell-free microDNA derived from uniquely mapping regions of the genome is detectable in plasma and serum from both mice and humans and that they are significantly longer (30-60% >250 bases) than cell-free circulating linear DNA (~150 bases). Tumor-derived human microDNA is detected in the mouse circulation in a mouse xenograft model of human ovarian cancer. Comparing the microDNA from paired tumor and normal lung tissue specimens reveals that the tumors contain longer microDNA. Consistent with human cancers releasing microDNA into the circulation, serum and plasma samples (12 lung and 11 ovarian cancer) collected prior to surgery are enriched for longer cell free microDNA compared to samples from the same patient obtained several weeks after surgical resection of the tumor. Thus, circular DNA in the circulation is a previously unexplored pool of nucleic acids that could complement microRNAs (miRs) and linear DNA for diagnosis and for intercellular communication. Extrachomosomal circular DNA (eccDNA) derived from chromosomal genomic sequence, first discovered in the nuclei of cells, are detected in the circulation, are longer than linear cell free DNA and are released from normal tissue and tumors into the circulation. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 174 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 174 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 32 | 18% |
| Researcher | 27 | 16% |
| Student > Bachelor | 17 | 10% |
| Student > Master | 15 | 9% |
| Student > Doctoral Student | 10 | 6% |
| Other | 33 | 19% |
| Unknown | 40 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 67 | 39% |
| Agricultural and Biological Sciences | 20 | 11% |
| Medicine and Dentistry | 17 | 10% |
| Unspecified | 5 | 3% |
| Neuroscience | 4 | 2% |
| Other | 18 | 10% |
| Unknown | 43 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 January 2024.
All research outputs
#4,512,313
of 24,293,076 outputs
Outputs from Molecular Cancer Research
#291
of 1,971 outputs
Outputs of similar age
#74,655
of 320,035 outputs
Outputs of similar age from Molecular Cancer Research
#5
of 35 outputs
Altmetric has tracked 24,293,076 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,971 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 320,035 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.