| Title |
Engineered jadomycin analogues with altered sugar moieties revealing JadS as a substrate flexible O-glycosyltransferase
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, April 2017
|
| DOI | 10.1007/s00253-017-8256-y |
| Pubmed ID | |
| Authors |
Liyuan Li, Guohui Pan, Xifen Zhu, Keqiang Fan, Wubin Gao, Guomin Ai, Jinwei Ren, Mingxin Shi, Carlos Olano, José A. Salas, Keqian Yang |
| Abstract |
Glycosyltransferases (GTs)-mediated glycodiversification studies have drawn significant attention recently, with the goal of generating bioactive compounds with improved pharmacological properties by diversifying the appended sugars. The key to achieving glycodiversification is to identify natural and/or engineered flexible GTs capable of acting upon a broad range of substrates. Here, we report the use of a combinatorial biosynthetic approach to probe the substrate flexibility of JadS, the GT in jadomycin biosynthesis, towards different non-native NDP-sugar substrates, enabling us to identify six jadomycin B analogues with different sugar moieties. Further structural engineering by precursor-directed biosynthesis allowed us to obtain 11 new jadomycin analogues. Our results for the first time show that JadS is a flexible O-GT that can utilize both L- and D- sugars as donor substrates, and tolerate structural changes at the C2, C4 and C6 positions of the sugar moiety. JadS may be further exploited to generate novel glycosylated jadomycin molecules in future glycodiversification studies. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 27% |
| Student > Ph. D. Student | 2 | 18% |
| Professor > Associate Professor | 2 | 18% |
| Student > Master | 1 | 9% |
| Other | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 2 | 18% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 36% |
| Biochemistry, Genetics and Molecular Biology | 3 | 27% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 9% |
| Chemistry | 1 | 9% |
| Design | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 1 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 January 2018.
All research outputs
#6,616,865
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#2,336
of 8,034 outputs
Outputs of similar age
#100,395
of 313,880 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#22
of 100 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,880 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 100 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.