| Title |
Reduced CaM Kinase II and CaM Kinase IV Activities Underlie Cognitive Deficits in NCKX2 Heterozygous Mice
|
|---|---|
| Published in |
Molecular Neurobiology, May 2017
|
| DOI | 10.1007/s12035-017-0596-1 |
| Pubmed ID | |
| Authors |
Shigeki Moriguchi, Satomi Kita, Yasushi Yabuki, Ryo Inagaki, Hisanao Izumi, Yuzuru Sasaki, Hideaki Tagashira, Kyoji Horie, Junji Takeda, Takahiro Iwamoto, Kohji Fukunaga |
| Abstract |
Among five members of the K(+)-dependent Na(+)/Ca(2+) exchanger (NCKX) family (NCKX1-5), only NCKX2 is highly expressed in mouse brain. NCKX2 in plasma membranes mediates cytosolic calcium excretion through electrogenic exchange of 4 Na(+) for 1 Ca(2+) and 1 K(+). Here, we observed significantly decreased levels of NCKX2 protein and mRNA in the CA1 region of APP23 mice, a model of Alzheimer's disease. We also found that, like APP23 mice, heterozygous NCKX2-mutant mice exhibit mildly impaired hippocampal LTP and memory acquisition, the latter based on novel object recognition and passive avoidance tasks. When we addressed underlying mechanisms, we found that both CaMKII autophosphorylation and CaMKIV phosphorylation significantly decreased in CA1 regions of NCKX2+/- relative to control mice. Likewise, phosphorylation of GluA1 (Ser-831) and CREB (Ser-133), respective downstream targets of CaMKII and CaMKIV, also significantly decreased in the CA1 region. BDNF protein and mRNA levels significantly decreased in CA1 of NCKX2+/- relative to control mice. Finally, CaN activity increased in CA1 of NCKX2+/- mice. Our findings suggest that like APP23 mice, NCKX2+/- mice may exhibit impaired learning and hippocampal LTP due to decreased CaM kinase II and CaM kinase IV activities. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 19% |
| Student > Master | 3 | 14% |
| Student > Bachelor | 2 | 10% |
| Unspecified | 1 | 5% |
| Professor | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 9 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 4 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 10% |
| Unspecified | 1 | 5% |
| Nursing and Health Professions | 1 | 5% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 10 | 48% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2021.
All research outputs
#3,148,241
of 22,977,819 outputs
Outputs from Molecular Neurobiology
#606
of 3,481 outputs
Outputs of similar age
#59,870
of 313,682 outputs
Outputs of similar age from Molecular Neurobiology
#18
of 124 outputs
Altmetric has tracked 22,977,819 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,481 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.2. This one has done well, scoring higher than 80% of its peers.
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We're also able to compare this research output to 124 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.